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Technical note: Viability and motility of vitrified/thawed primordial germ cell isolated from common carp (Cyprinus carpio) somite embryos

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Title: Technical note: Viability and motility of vitrified/thawed primordial germ cell isolated from common carp (Cyprinus carpio) somite embryos
Authors: Kawakami, Y. Browse this author
Saito, T. Browse this author
Fujimoto, T. Browse this author
Goto-Kazeto, R. Browse this author
Takahashi, E. Browse this author
Adachi, S. Browse this author
Arai, K. Browse this author →KAKEN DB
Yamaha, E. Browse this author →KAKEN DB
Keywords: common carp
germ-line chimera
goldfish
primordial germ cell
transplantation
vitrification
Issue Date: Feb-2012
Publisher: American Society of Animal Science
Journal Title: Journal of Animal Science
Volume: 90
Issue: 2
Start Page: 495
End Page: 500
Publisher DOI: 10.2527/jas.2011-4329
PMID: 21926320
Abstract: The feasibility of cryopreserving common carp (Cyprinus carpio) primordial germ cells (PGC) by vitrification of whole embryos at the 22 to 28 somite stage was investigated. Green fluorescent protein (GFP)-labeled PGC were cooled rapidly using liquid nitrogen after exposure to a pretreatment solution containing 1.5 M cryoprotectant (ethylene glycol or dimethyl sulfoxide, 30 or 50 min) and a vitrification solution containing 3 M cryoprotectant and 0.5 M sucrose (5, 10, 20, or 30 min). Embryonic cells that were pretreated for 30 min and vitrified for 20 min with ethylene glycol had the greatest rate of survival of embryonic cells (68.6%; P < 0.01), an optimal highest percentage of viable PGC (73.8% to 74.9%; P < 0.05), and no evidence of ice formation after thawing. The vitrified/ thawed PGC were transplanted into blastula-stage embryos from goldfish (Carassius auratus). The PGC maintained their motility and moved to the gonadal ridge of the host embryo. Thus, the combination of vitrification and transplantation to produce germ-line chimeras is a powerful tool for the artificial production of next-generation offspring.
Type: article (author version)
URI: http://hdl.handle.net/2115/51756
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川上 優

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