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A designed curved DNA sequence remarkably enhances transgene expression from plasmid DNA in mouse liver.

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Title: A designed curved DNA sequence remarkably enhances transgene expression from plasmid DNA in mouse liver.
Authors: Fukunaga, S. Browse this author
Kanda, G. Browse this author
Tanase, J. Browse this author
Harashima, H. Browse this author →KAKEN DB
Ohyama, T. Browse this author
Kamiya, H. Browse this author →KAKEN DB
Keywords: left-handedly curved DNA
exogenous DNA
intranuclear disposition
Issue Date: Aug-2012
Publisher: Nature Publishing
Journal Title: Gene therapy
Volume: 19
Issue: 8
Start Page: 828
End Page: 835
Publisher DOI: 10.1038/gt.2011.127
PMID: 21918548
Abstract: The intranuclear disposition of plasmid DNA is extremely important for transgene expression. The interactions between the plasmid DNA and the histone proteins are one of the keys for controlling the disposition. In this study, the effects of a left-handedly curved sequence (20-40 repeated A•T tracts) on transgene expression from a plasmid were examined in vivo. A naked luciferase plasmid with the curved sequence was delivered into mouse liver by a hydrodynamics-based injection, and the luciferase activities were quantitated at various time points. Interestingly, transgene expression was markedly increased by the addition of the curved sequence. An analysis of the nucleosome positions near the left-handedly curved sequence suggested that the sequence functions as an acceptor of the histone core and allows nucleosome sliding, resulting in transcriptional activation. These results suggested that the designed curved DNA sequences could control transgene expression from plasmid DNAs in vivo.
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷 浩之

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