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Species Differences in the Distribution of the Nonmuscle Myosin Heavy Chain IIB Inserted Isoform in the Brain
| Title: | Species Differences in the Distribution of the Nonmuscle Myosin Heavy Chain IIB Inserted Isoform in the Brain |
| Authors: | Hagiwara, Shin-ya Browse this author | | Takahashi, Masayuki Browse this author | | Yamagishi, Akihiko Browse this author |
| Issue Date: | 2001 |
| Publisher: | Zoological Society of Japan |
| Journal Title: | Zoological Science |
| Volume: | 18 |
| Issue: | 1 |
| Start Page: | 43 |
| End Page: | 49 |
| Publisher DOI: | 10.2108/zsj.18.43 |
| Abstract: | The alternatively spliced isoform of the nonmuscle myosin heavy chain IIB (MHC-IIB) with an insert of 21 amino acids near the actin-binding region, MHC-IIB(B2), is expressed specifically in the brain and spinal cord in Mammalia and Aves. We performed immunoblot analyses to elucidate the distribution of MHC-IIB(B2) in the brains of various animals. Nearly half of MHC-IIB existed as the B2 inserted isoform (MHC-IIB(B2)) in the cerebrum of the guinea-pig, rabbit and pig, while the non-B2 inserted isoform (MHCIIB(ΔB2)) was the dominant form in the cerebrum of the rat, mouse and hamster. In the guinea-pig, the amount of MHC-IIB(B2) expressed in the cerebrum was low compared to MHC-IIB(ΔB2) during the first postnatal week, but it increased to comparable levels during postnatal development. In the rat, the amount of MHC-IIB(B2) protein in the cerebrum remains low compared to its expression elsewhere in the brain throughout life. Our results regarding the distribution of MHC-IIB(B2) in the adult brain lead us to classify species into two types; one type expresses significantly less MHC-IIB(B2) in the cerebrum than in other portions of the brain, and the second type expresses it at comparable levels throughout the brain. Based on these results, we hypothesize that MHC-IIB(B2) modulates the role of MHC-IIB(ΔB2) in the regulation of synaptic structure and function in the mature brain, and that the requirements for such function by MHC-IIB(B2) have shifted gradually from the cerebellum to the cerebrum with evolutionary increases in brain size. |
| Relation: | http://www.zoology.or.jp/html/02_magazine/02_index.htm |
| Type: | article |
| URI: | http://hdl.handle.net/2115/52963 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高橋 正行
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