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養殖サケ科魚類の人工採卵における等調液洗卵法の除菌効果

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Title: 養殖サケ科魚類の人工採卵における等調液洗卵法の除菌効果
Other Titles: Effect of egg-washing with isotonic solution on elimination of bacteria and viruses before fertilization in salmon aquaculture
Authors: 小原, 昌和1 Browse this author
小川, 滋2 Browse this author
笠井, 久会3 Browse this author →KAKEN DB
吉水, 守4 Browse this author →KAKEN DB
Authors(alt): Kohara, Masakazu1
Ogawa, Shigeru2
Kasai, Hisae3
Yoshimizu, Mamoru4
Keywords: Egg-washing
Isotonic solution
Intra-ovum infection
Flavobacterium psychrophilum
Issue Date: 20-Mar-2010
Publisher: 日本水産増殖学会
Journal Title: 水産増殖
Journal Title(alt): The aquiculture
Volume: 58
Issue: 1
Start Page: 37
End Page: 43
Publisher DOI: 10.11233/aquaculturesci.58.37
Abstract: 冷水病菌,せっそう病菌,IHNVおよびOMVで汚染したニジマス卵を供試して,等調液洗卵の除菌効果を定量的に明らかにするとともに,ニジマス卵を使用した感染試験を行い,卵内感染の防止効果について検証した。その結果,冷水病菌,せっそう病菌ではそれぞれ10(3)CFU/ml,10(5)CFU/ml,IHNVおよびOMVについては10(4)TCID50/ml程度の菌体あるいはウイルスが等調液洗卵により除去されることが明らかとなった。また,シャワー洗卵においては,等調液量を増加させることにより,冷水病菌およびせっそう病菌の除菌量が増加した。さらに,冷水病菌で高度に汚染した卵を濯ぎ洗卵した場合には,卵内感染の割合が低くなり,シャワー洗卵をした卵では卵内感染卵は検出されなかった。これらの結果から,等調液洗卵法は卵表面に存在する細菌,ウイルスの除菌あるいは除去効果があり,卵内感染のリスクを小さくするために有効な方法であることが示された。
Egg-washing with isotonic solution has been used to improve the artificial fertilization in fresh-water salmon aquaculture. Usually, unfertilized eggs are rinsed twice or three times with isotonic solution and showered before fertilization. We investigated the effect of egg-washing using rainbow trout eggs on elimination of some causative agents of cold water disease (Flavobacterium psychrophilum), flunculosis (Aeromonas salmonicida), infectious hematopoietic necrosis (IHNV) and Oncorhynchus masou virus disease (OMV), quantitatively and on prevention of intra-ovum infection with F. psychrophilum. The number of live bacterial cells or virus infectivity titers which were eliminated by egg-washing, was 10(3)CFU/ml in F. psychrophilum, 10(5)CFU/ml in A. salmonicida, or 10(4)TCID50/ml in both of IHNV and OMV. In shower washing, the eliminated extent of F. psychrophilum or A. salmonicida cells increased with increasing isotonic solution volume. The ratio of intra-ovum infection with F. psychrophilum was clearly less in rinsed eggs than in non-treated eggs, and result of detection from showered eggs was negative. These results suggest that egg-washing with isotonic solution is very effective to eliminate the bacteria or viruses on egg surface and is able to decrease the risk of intra-ovum infection.
Rights: © 2010 日本水産増殖学会
Type: article
URI: http://hdl.handle.net/2115/53097
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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