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大腸菌発現VNNウイルス外被タンパク質に対するマツカワの液性免疫応答開始時期

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Title: 大腸菌発現VNNウイルス外被タンパク質に対するマツカワの液性免疫応答開始時期
Other Titles: Age-dependent humoral immune response of barfin flounder against recombinant coat protein of barfin flounder nervous necrosis virus
Authors: 渡辺, 研一1 Browse this author
吉水, 守2 Browse this author →KAKEN DB
Authors(alt): Watanabe, Ken-ichi1
Yoshimizu, Mamoru2
Keywords: Betanodavirus
BFNNV
Immune response
Verasper moseri
ELISA antibody titer
Barfin flounder
Issue Date: 15-Jun-2002
Publisher: 日本魚病学会
Journal Title: 魚病研究
Journal Title(alt): Fish pathology
Volume: 37
Issue: 2
Start Page: 92
End Page: 94
Publisher DOI: 10.3147/jsfp.37.92
Abstract: Age-dependent humoral immune response of barfin flounder Verasper moseri was investigated. One hundred μL of recombinant coat protein of barfin flounder nervous necrosis virus (BFNNV) adjusted to 250 μg/mL in Tris-EDTA buffer (pH 8.0) was injected intravascularly to 4, 5, 6 and 7 month-old fish. One month after injection, an enzyme-linked immunosorbent assay (ELISA) antibody titer was measured. Although no ELISA antibody titers were detected in injected or noninjected fish of 4 and 5 month-old, the antibody titers were remarkably increased (p<0.025) in 6 and 7 month-old fish. High ELISA antibody titers were kept for at least 2 months.
マツカワの液性免疫応答開始時期の検討を行った。 4~7ケ月齢魚の尾部血管内に250μg/mlのタンパク質量に調整した大腸菌発現 BFNNV 外被タンパク質(32kDa) を 100μL注射した。 抗原投与後1ヶ月毎に ELISA を用いて血清中の抗体価を測定した。 5ヶ月齢以下の魚では投与の前後で ELISA 抗体価に違いは認められなかったが, 6ヶ月齢以降の魚では明瞭な ELISA 抗体価の上昇が観察された。 高いELISA 抗体価は抗原投与2ヶ月間にわたって維持された。
Type: article
URI: http://hdl.handle.net/2115/53157
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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