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Title: 細胞培養法とRT-PCR法を組み合わせた神経壊死症ウイルスの検出
Other Titles: Detection of nervous necrosis virus using RT-PCR combined with tissue culture
Authors: 渡辺, 研一1 Browse this author
吉水, 守2 Browse this author →KAKEN DB
Authors(alt): Watanabe, Ken-ichi1
Yoshimizu, Mamoru2
Keywords: ウイルス性神経壊死症
Issue Date: 15-Mar-2002
Publisher: 日本水産学会
Journal Title: 日本水産学会誌
Journal Title(alt): Bulletin of the Japanese Society of Scientific Fisheries
Volume: 68
Issue: 2
Start Page: 197
End Page: 200
Publisher DOI: 10.2331/suisan.68.197
Abstract: 高感度で迅速な神経壊死症原因ウイルス(NNV)検出法を開発するために,試料を直接PCRする方法,培養細胞を用いる方法,および両者の併用法のウイルス検出結果を比較した。材料としてシマアジ,マダラ,マガレイおよびヒラメのVNN罹病仔稚魚を用いた。併用法(7日間予備培養+PCR)の検出感度は,直接PCR法より2〜5オーダー,ウイルス分離法より2〜5オーダー(観察期間7日),あるいは0〜2オーダー(観察期間14日)高かった。このことから,試料接種後7日間予備培養したSSN-1細胞をPCR法により検査する方法が,最も高感度に,かつ迅速に感染性を持つVNNウイルスを検出できる方法であることが明らかになった。
The detection rates of viral nervous necrosis (VNN) virus by direct RT-PCR, tissue culture using SSN-1 cell line and RT-PCR combined with tissue culture were compared. Tested specimens were whole bodies of striped jack and brown sole larvae, eyes and brain of Pacific cod juveniles, and heads of Japanese flounder juveniles, which were affected by VNN. The specimens were diluted with a 10-fold serial dilution. RT-PCR combined with tissue culture for seven days using the SSN-1 cell line was the most sensitive and rapid method for the detection of infectious VNN virus.
Rights: © 2002 公益社団法人日本水産学会
© 2002 The Japanese Society of Fisheries Science
Type: article
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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