HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
薬学研究院  >
雑誌発表論文等  >

Simian virus 40 DNA replication in vitro: purification and characterization of replication factors from mouse cells.

フルテキスト
19_Ariga_1986_Nucleic_Acids_Res.pdf1.5 MBPDF見る/開く
この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/54050

タイトル: Simian virus 40 DNA replication in vitro: purification and characterization of replication factors from mouse cells.
著者: Ariga, Hiroyoshi 著作を一覧する
発行日: 1986年12月 9日
出版者: Oxford University Press
誌名: Nucleic acids research
巻: 14
号: 23
開始ページ: 9457
終了ページ: 9470
出版社 DOI: 10.1093/nar/14.23.9457
抄録: We have previously developed simian virus 40 (SV40) DNA replication system in vitro (Ariga and Sugano, J. Virol. 48, 481, 1983). This system is composed of human HeLa or mouse FM3A nuclear extract and cytoplasmic extract of SV40 infected CosI cells. Here FM3A nuclear extract was fractionated by DEAE Sephacel and single-stranded DNA cellulose chromatography into three components required for accurate in vitro SV40 DNA replication. One fraction (A fraction) contained DNA polymerase-primase, and the second component (B fraction) contained DNA topoisomerase. Third component was further purified to near homogenuity using DEAE-Sephacel, single-stranded DNA cellulose, and glycerol gradient centrifugation. The purified protein (named factor I) bound to the origin containing fragment of SV40 DNA. The factor I enhanced the initiation of SV40 DNA replication catalyzed by SV40 infected CosI cytoplasm alone. When all four fractions consisting of A, B fractions, factor I, and SV40 infected CosI cytoplasm were mixed together, the system was reconstituted, meaning that initiation and subsequent elongation were completed to generate the full sized daughter molecules.
Relation (URI): http://nar.oxfordjournals.org/content/14/23/9457.abstract
資料タイプ: article
URI: http://hdl.handle.net/2115/54050
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 有賀 寛芳

 

本サイトに関するご意見・お問い合わせは repo at lib.hokudai.ac.jp へお願いします。 - 北海道大学