HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >

Polymerase complex with lysine at position 627 of the PB2 of influenza virus A/Hong Kong/483/97 (H5N1) efficiently transcribes and replicates virus genes in mouse cells

Files in This Item:
VR revised manuscript (Yamamoto130731).pdf461.49 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/54759

Title: Polymerase complex with lysine at position 627 of the PB2 of influenza virus A/Hong Kong/483/97 (H5N1) efficiently transcribes and replicates virus genes in mouse cells
Authors: Yamamoto, Naoki Browse this author
Sakoda, Yoshihiro Browse this author →KAKEN DB
Okamatsu, Masatoshi Browse this author →KAKEN DB
Kida, Hiroshi Browse this author →KAKEN DB
Keywords: PB2
H5N1
Influenza A virus
Polymerase
Issue Date: 26-Dec-2013
Publisher: Elsevier
Journal Title: Virus Research
Volume: 178
Issue: 2
Start Page: 404
End Page: 410
Publisher DOI: 10.1016/j.virusres.2013.09.012
PMID: 24070983
Abstract: Influenza virus A/Hong Kong/483/97 (H5N1) (HK483-K) has the PB2 with lysine at position 627 (PB2-627K) and is highly pathogenic in chickens and mice. On the other hand, the pathogenicity of mutant virus (HK483-E), which was generated by substituting lysine with glutamic acid at the position of the PB2, is lower than that of HK483-K in mice, but is highly pathogenic in chickens. The PB2 is one of the components of heterotrimeric polymerase complex, which plays roles in the transcription and replication of virus genes. Cell-free polymerase assay revealed that intrinsic transcription activity of the polymerase complex with PB2-627K is higher than that of glutamic acid (PB2-627E). In chicken cells, transcription efficiency of the polymerase complex with PB2-627E was not lower than those with PB2-627K, indicating that transcription of virus genes is modulated by some host factors in chicken cells, resulting in high growth. Polymerase complex with PB2-627K efficiently transcribes and replicates virus polymerase genes in mouse cells, leading to high growth of HK483-K compared with that of HK483-E. The results of our experiments clearly suggest that efficient transcription and replication of virus genes by polymerase complex result in the higher pathogenicity in mice. (C) 2013 Elsevier B.V. All rights reserved.
Type: article (author version)
URI: http://hdl.handle.net/2115/54759
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 迫田 義博

Export metadata:

OAI-PMH ( junii2 , jpcoar )


 

Feedback - Hokkaido University