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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Viral envelope protein glycosylation is a molecular determinant of the neuroinvasiveness of the New York strain of West Nile virus
| Title: | Viral envelope protein glycosylation is a molecular determinant of the neuroinvasiveness of the New York strain of West Nile virus |
| Authors: | Shirato, Kazuya Browse this author | | Miyoshi, Hirotsugu Browse this author | | Goto, Akiko Browse this author | | Ako, Yoshihiko Browse this author | | Ueki, Tomotaka Browse this author | | Kariwa, Hiroaki Browse this author →KAKEN DB | | Takashima, Ikuo7 Browse this author →KAKEN DB |
| Authors(alt): | 高島, 郁夫7 |
| Issue Date: | 2004 |
| Publisher: | Society for General Microbiology |
| Journal Title: | Journal of General Virology |
| Volume: | 85 |
| Start Page: | 3637 |
| End Page: | 3645 |
| Publisher DOI: | 10.1099/vir.0.80247-0 |
| PMID: | 15557236 |
| Abstract: | Two New York (NY) strains of the West Nile (WN) virus were plaque-purified and four variants that had different amino acid sequences at the N-linked glycosylation site in the envelope (E) protein sequence were isolated. The E protein was glycosylated in only two of these strain variants. To determine the relationship between E protein glycosylation and pathogenicity of the WN virus, 6-week-old mice were infected subcutaneously with these variants. Mice infected with viruses that carried the glycosylated E protein developed lethal infection, whereas mice infected with viruses that carried the non-glycosylated E protein showed low mortality. In contrast, intracerebral infection of mice with viruses carrying either the glycosylated or non-glycosylated forms of the E protein resulted in lethal infection. These results suggested that E protein glycosylation is a molecular determinant of neuroinvasiveness in the NY strains of WN virus. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/5569 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高島 郁夫
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