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LIM-homeodomain transcription factor Awh is a key component activating all three fibroin genes, fibH, fibL and fhx, in the silk gland of the silkworm, Bombyx mori

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タイトル: LIM-homeodomain transcription factor Awh is a key component activating all three fibroin genes, fibH, fibL and fhx, in the silk gland of the silkworm, Bombyx mori
著者: Kimoto, Mai 著作を一覧する
Tsubota, Takuya 著作を一覧する
Uchino, Keiro 著作を一覧する
Sezutsu, Hideki 著作を一覧する
Takiya, Shigeharu 著作を一覧する
キーワード: Bombyx mori
Silk gland
Fibroin
Awh
Transgenic
発行日: 2015年 1月
出版者: Elsevier
誌名: Insect biochemistry and molecular biology
巻: 56
開始ページ: 29
終了ページ: 35
出版社 DOI: 10.1016/j.ibmb.2014.11.003
抄録: In the silkworm Bombyx mori, three fibroin genes, fibroin-heavy-chain (fibH), fibroin-light-chain (fibL) and fibrohexamerin (fhx), are coexpressed only in the posterior silk gland (PSG) cells, while the sericin genes encoding silk glue proteins are expressed in the middle silk gland (MSG) cells. Silk gland factor-2 (SGF-2) is a PSG-specific activator complex of fibH, composed of a LIM-homeodomain protein, Awh, and its cofactors, Ldb and Lcaf. We investigated whether SGF-2 can activate other fibroin genes using transgenic silkworms. The genes for Ldb and Lcaf were expressed ubiquitously in various tissues, while the gene for Awh was expressed strictly specific in PSG of the wild type silkworms. Misexpression of Awh in transgenic silkworms induced ectopic expression of fibL and fhx as well as fibH in MSG. Coincidently with the induction of fibL and fhx by Awh, binding of SGF-2 to the promoter of fibL and fhx was detected in vitro, and SGF-2 binds directly to the fhx core promoter. Ectopic expression of the fibroin genes was observed at high levels in the middle part of MSG. Moreover, fibL and fhx were induced in the anterior silk gland (ASG) of the transgenic silkworms, but fibH was not. These results indicate that Awh is a key activator of all three fibroin genes, and the activity is probably regulated in conjunction with additional factors.
Rights: (C) 2014 Elsevier Ltd. All rights reserved.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/58056
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 瀧谷 重治

 

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