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Temperature adaptation in Dictyostelium: role of Δ5 fatty acid desaturase

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Title: Temperature adaptation in Dictyostelium: role of Δ5 fatty acid desaturase
Authors: Saito, Tamao1 Browse this author →KAKEN DB
Kato, Atsushi Browse this author →KAKEN DB
Ochiai, Hiroshi Browse this author →KAKEN DB
Morita, Naoki Browse this author
Authors(alt): 齊藤, 玉緒1
Issue Date: 2005
Publisher: Society for General Microbiology
Journal Title: Microbiology
Volume: 151
Start Page: 113
End Page: 119
Publisher DOI: 10.1099/mic.0.27651-0
PMID: 15632431
Abstract: Membrane fluidity is critical for proper membrane function and is regulated in part by the proportion of unsaturated fatty acids present in membrane lipids. The proportion of these lipids in turn varies with temperature and may contribute to temperature adaptation in poikilothermic organisms. The fundamental question posed in this study was whether the unsaturation of fatty acids contributes to the ability to adapt to temperature stress in Dictyostelium. First, fatty acid composition was analysed and it was observed that the relative proportions of dienoic acids changed with temperature. To investigate the role of dienoic fatty acids in temperature adaptation, null mutants were created in the two known Δ5 fatty acid desaturases (FadA and FadB) that are responsible for the production of dienoic fatty acids. The fadB null mutant showed no significant alteration in fatty acid composition or in phenotype. However, the disruption of fadA resulted in a large drop in dienoic fatty acid content from 51・2 to 4・1% and a possibly compensatory increase in monoenoic fatty acids (40・9–92・4 %). No difference was detected in temperature adaptation with that of wild-type cells during the growth phase. However, surprisingly, mutant cells developed more efficiently than the wild-type at elevated temperatures. These results show that the fatty acid composition of Dictyostelium changes with temperature and suggest that the regulation of dienoic fatty acid synthesis is involved in the development of Dictyostelium at elevated temperatures, but not during the growth phase.
Type: article
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 齊藤 玉緒

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