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Mitochondrial activity during pre-maturational culture in in vitro-grown bovine oocytes is related to maturational and developmental competences.

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/59542

Title: Mitochondrial activity during pre-maturational culture in in vitro-grown bovine oocytes is related to maturational and developmental competences.
Authors: Huang, Weiping Browse this author
Kang, Sung-Sik Browse this author
Nagai, Katsuhisa Browse this author
Yanagawa, Yojiro Browse this author
Takahashi, Yoshiyuki Browse this author →KAKEN DB
Nagano, Masashi Browse this author →KAKEN DB
Keywords: early antral follicle
embryo transfer
IVG
mitochondria
ROS
Issue Date: 17-Jul-2014
Publisher: Society for Reproduction and Fertility
Journal Title: Reproduction, fertility, and development
Volume: 28
Issue: 3
Start Page: 349
End Page: 356
Publisher DOI: 10.1071/RD14023
PMID: 25029376
Abstract: The objective of this study was to investigate the dynamics of mitochondrial activity in in vitro-grown (IVG) bovine oocytes during pre-maturational culture (pre-IVM) and its relationship to their developmental competence upon being subjected to different pre-IVM durations. After 12-day IVG culture, oocytes were cultured for 0, 10 or 20 h with 3-isobutyl-1-methylxanthine (IBMX) as pre-IVM. Mitochondrial activity in IVG oocytes after 10 h pre-IVM was the highest among all the pre-IVM durations (P < 0.05). In addition, cleavage (79.4%) and blastocyst rates (38.9%) of embryos derived from IVG oocytes with 10 h pre-IVM were higher than those with 20 h pre-IVM (63.0 and 25.8%, respectively; P < 0.05) and similar to those of in vivo-grown oocytes (82.7 and 36.7%, respectively). To confirm the developmental ability of IVG oocytes with 10 h pre-IVM beyond the blastocyst stage in vivo, embryo transfer was attempted. Transferred embryos developed to the elongated embryonic stage (63.6%, 7/11) in the recipient uterus atDay 16 of oestrus, and a male calf was delivered (50%, 1/2). In conclusion, it was indicated that the mitochondrial activity of bovine IVG oocytes peaked at 10 h pre-IVM and was closely correlated with the nuclear maturation and developmental competences of IVG oocytes.
Rights: Disclaimer. This is not the definitive version of record of this article. This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, the journal accepts no responsibility for any errors or omissions it may contain. The definitive version is available at http://dx.doi.org/10.1071/RD14023 © 2014 Society for Reproduction and Fertility.
Type: article (author version)
URI: http://hdl.handle.net/2115/59542
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 永野 昌志

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