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Mitochondrial activity during pre-maturational culture in in vitro-grown bovine oocytes is related to maturational and developmental competences.
| Title: | Mitochondrial activity during pre-maturational culture in in vitro-grown bovine oocytes is related to maturational and developmental competences. |
| Authors: | Huang, Weiping Browse this author | | Kang, Sung-Sik Browse this author | | Nagai, Katsuhisa Browse this author | | Yanagawa, Yojiro Browse this author | | Takahashi, Yoshiyuki Browse this author →KAKEN DB | | Nagano, Masashi Browse this author →KAKEN DB |
| Keywords: | early antral follicle | | embryo transfer | | IVG | | mitochondria | | ROS |
| Issue Date: | 17-Jul-2014 |
| Publisher: | Society for Reproduction and Fertility |
| Journal Title: | Reproduction, fertility, and development |
| Volume: | 28 |
| Issue: | 3 |
| Start Page: | 349 |
| End Page: | 356 |
| Publisher DOI: | 10.1071/RD14023 |
| PMID: | 25029376 |
| Abstract: | The objective of this study was to investigate the dynamics of mitochondrial activity in in vitro-grown (IVG) bovine oocytes during pre-maturational culture (pre-IVM) and its relationship to their developmental competence upon being subjected to different pre-IVM durations. After 12-day IVG culture, oocytes were cultured for 0, 10 or 20 h with 3-isobutyl-1-methylxanthine (IBMX) as pre-IVM. Mitochondrial activity in IVG oocytes after 10 h pre-IVM was the highest among all the pre-IVM durations (P < 0.05). In addition, cleavage (79.4%) and blastocyst rates (38.9%) of embryos derived from IVG oocytes with 10 h pre-IVM were higher than those with 20 h pre-IVM (63.0 and 25.8%, respectively; P < 0.05) and similar to those of in vivo-grown oocytes (82.7 and 36.7%, respectively). To confirm the developmental ability of IVG oocytes with 10 h pre-IVM beyond the blastocyst stage in vivo, embryo transfer was attempted. Transferred embryos developed to the elongated embryonic stage (63.6%, 7/11) in the recipient uterus atDay 16 of oestrus, and a male calf was delivered (50%, 1/2). In conclusion, it was indicated that the mitochondrial activity of bovine IVG oocytes peaked at 10 h pre-IVM and was closely correlated with the nuclear maturation and developmental competences of IVG oocytes. |
| Rights: | Disclaimer. This is not the definitive version of record of this article. This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, the journal accepts no responsibility for any errors or omissions it may contain. The definitive version is available at http://dx.doi.org/10.1071/RD14023 © 2014 Society for Reproduction and Fertility. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/59542 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 永野 昌志
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