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Sugar Beet (Beta vulgaris L.)
Title: | Sugar Beet (Beta vulgaris L.) |
Authors: | Kagami, Hiroyo Browse this author | Kurata, Masayuki Browse this author | Matsuhira, Hiroaki Browse this author | Taguchi, Kazunori Browse this author | Mikami, Tetsuo Browse this author →KAKEN DB | Tamagake, Hideto Browse this author | Kubo, Tomohiko Browse this author →KAKEN DB |
Keywords: | Agrobacterium tumefaciens | Beta vulgaris | binary vector | callus | genetic transformation | regeneration | sugar beet |
Issue Date: | Sep-2014 |
Publisher: | Springer |
Journal Title: | Methods in Molecular Biology |
Volume: | 1223 |
Start Page: | 335 |
End Page: | 347 |
Publisher DOI: | 10.1007/978-1-4939-1695-5_27 |
PMID: | 25300853 |
Abstract: | Creating transgenic plants is invaluable for the genetic analysis of sugar beet and will be increasingly important as sugar beet genomic technologies progress. A protocol for Agrobacterium-mediated transformation of sugar beet is described in this chapter. Our protocol is optimized for a sugar beet genotype that performs exceptionally well in tissue culture, including the steps of dedifferentiation, callus proliferation, and regeneration. Because of the infrequent occurrence of such a genotype in sugar beet populations, our protocol includes an in vitro propagation method for germplasm preservation. The starting materials for transgenic experiments are aseptic shoots grown from surface-sterilized seed balls. Callus is induced from leaf explants and subsequently infected with Agrobacterium. Plantlets are regenerated from transgenic callus and vernalized for flowering, if necessary. The efficiency of transformation was quite high; in our laboratory, the culture of only 10 leaf explants, on average, generated one transgenic plant. |
Rights: | The final publication is available at www.springerlink.com |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/59861 |
Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 久保 友彦
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