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Sugar Beet (Beta vulgaris L.)

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Title: Sugar Beet (Beta vulgaris L.)
Authors: Kagami, Hiroyo Browse this author
Kurata, Masayuki Browse this author
Matsuhira, Hiroaki Browse this author
Taguchi, Kazunori Browse this author
Mikami, Tetsuo Browse this author →KAKEN DB
Tamagake, Hideto Browse this author
Kubo, Tomohiko Browse this author →KAKEN DB
Keywords: Agrobacterium tumefaciens
Beta vulgaris
binary vector
genetic transformation
sugar beet
Issue Date: Sep-2014
Publisher: Springer
Journal Title: Methods in Molecular Biology
Volume: 1223
Start Page: 335
End Page: 347
Publisher DOI: 10.1007/978-1-4939-1695-5_27
PMID: 25300853
Abstract: Creating transgenic plants is invaluable for the genetic analysis of sugar beet and will be increasingly important as sugar beet genomic technologies progress. A protocol for Agrobacterium-mediated transformation of sugar beet is described in this chapter. Our protocol is optimized for a sugar beet genotype that performs exceptionally well in tissue culture, including the steps of dedifferentiation, callus proliferation, and regeneration. Because of the infrequent occurrence of such a genotype in sugar beet populations, our protocol includes an in vitro propagation method for germplasm preservation. The starting materials for transgenic experiments are aseptic shoots grown from surface-sterilized seed balls. Callus is induced from leaf explants and subsequently infected with Agrobacterium. Plantlets are regenerated from transgenic callus and vernalized for flowering, if necessary. The efficiency of transformation was quite high; in our laboratory, the culture of only 10 leaf explants, on average, generated one transgenic plant.
Rights: The final publication is available at
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 久保 友彦

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