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Interploid and intraploid hybridizations to produce polyploid Haskap (Lonicera caerulea var. emphyllocalyx) plants

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Title: Interploid and intraploid hybridizations to produce polyploid Haskap (Lonicera caerulea var. emphyllocalyx) plants
Authors: Miyashita, Tomomi Browse this author
Hoshino, Yoichiro Browse this author →KAKEN DB
Keywords: Caprifoliaceae
Interploid cross
Intraploid cross
Lonicera caerulea
Haskap
Polyploid
Issue Date: Jan-2015
Publisher: Springer
Journal Title: Euphytica
Volume: 201
Issue: 1
Start Page: 15
End Page: 27
Publisher DOI: 10.1007/s10681-014-1159-4
Abstract: We produced polyploid Haskap (Lonicera caerulea var. emphyllocalyx) plants by performing interploid and intraploid crosses of wild accessions. Embryo rescue in a tetraploid (4x) × diploid (2x) cross produced triploid plants; reciprocal 2x × 4x cross failed to produce viable seeds. Intraploidy crosses of 4x × 4x produced mostly tetraploids but also several hexaploid (6x) and octoploid (8x) plants. Using hexaploids obtained from this cross, we examined reciprocal 4x–6x crosses and found that both produced pentaploid plants. An octoploid was produced by applying colchicine to a tetraploid; a 4x × 8x cross using this plant and aided by embryo rescue culture produced three hexaploid plants, with an aneuploid number of chromosomes. Several plants obtained in this study flowered and set fruits. We discuss the overall efficiency of producing polyploid plants in interploid and intraploid crosses.
Rights: The final publication is available at link.springer.com
Type: article (author version)
URI: http://hdl.handle.net/2115/60407
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 星野 洋一郎

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