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The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/60734

Title: The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles
Authors: Nagai, Katsuhisa Browse this author
Yanagawa, Yojiro Browse this author
Katagiri, Seiji Browse this author →KAKEN DB
Nagano, Masashi Browse this author →KAKEN DB
Issue Date: 25-Feb-2016
Publisher: Biomedical Research Press
Journal Title: Biomedical Research
Volume: 37
Issue: 1
Start Page: 63
End Page: 71
Publisher DOI: 10.2220/biomedres.37.63
PMID: 26912141
Abstract: To clarify the relationship between ovarian reserve and the developmental competence of bovine oocytes, oocyte-granulosa complexes (OGCs) collected from early antral follicles (≤1 mm) in ovaries with high (≥25) and low (<25) antral follicle counts (AFCs) were used. OGCs derived from different AFC groups were cultured for growth followed by maturation, fertilization and blastocyst formation. Viability of OGCs during growth culture was similar between groups; however, OGCs in the high-AFC group had a larger number of granulosa cells than the low-AFC group at 12 days of growth. The proportion of matured oocytes in the high-AFC group was higher than that in the low-AFC group. Mitochondrial activity of oocytes before maturation in the high-AFC group was higher than that in the low-AFC group; however, accumulation of reactive oxygen species was similar between groups. Cleavage rate in the high-AFC group tended to be higher than that in the low-AFC group, although blastocyst development was similar between groups. In conclusion, oocytes derived from ovaries with high AFC have higher maturational ability and fertilizability than those from low AFC. The difference may be caused by high proliferation of granulosa cells from ovaries with high AFC.
Type: article
URI: http://hdl.handle.net/2115/60734
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 永野 昌志

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