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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles
| Title: | The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles |
| Authors: | Nagai, Katsuhisa Browse this author | | Yanagawa, Yojiro Browse this author | | Katagiri, Seiji Browse this author →KAKEN DB | | Nagano, Masashi Browse this author →KAKEN DB |
| Issue Date: | 25-Feb-2016 |
| Publisher: | Biomedical Research Press |
| Journal Title: | Biomedical Research |
| Volume: | 37 |
| Issue: | 1 |
| Start Page: | 63 |
| End Page: | 71 |
| Publisher DOI: | 10.2220/biomedres.37.63 |
| PMID: | 26912141 |
| Abstract: | To clarify the relationship between ovarian reserve and the developmental competence of bovine oocytes, oocyte-granulosa complexes (OGCs) collected from early antral follicles (≤1 mm) in ovaries with high (≥25) and low (<25) antral follicle counts (AFCs) were used. OGCs derived from different AFC groups were cultured for growth followed by maturation, fertilization and blastocyst formation. Viability of OGCs during growth culture was similar between groups; however, OGCs in the high-AFC group had a larger number of granulosa cells than the low-AFC group at 12 days of growth. The proportion of matured oocytes in the high-AFC group was higher than that in the low-AFC group. Mitochondrial activity of oocytes before maturation in the high-AFC group was higher than that in the low-AFC group; however, accumulation of reactive oxygen species was similar between groups. Cleavage rate in the high-AFC group tended to be higher than that in the low-AFC group, although blastocyst development was similar between groups. In conclusion, oocytes derived from ovaries with high AFC have higher maturational ability and fertilizability than those from low AFC. The difference may be caused by high proliferation of granulosa cells from ovaries with high AFC. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/60734 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 永野 昌志
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