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The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles

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Biomed Res v.37p.63.pdf603.36 kBPDF見る/開く
この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/60734

タイトル: The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles
著者: Nagai, Katsuhisa 著作を一覧する
Yanagawa, Yojiro 著作を一覧する
Katagiri, Seiji 著作を一覧する
Nagano, Masashi 著作を一覧する
発行日: 2016年 2月25日
出版者: Biomedical Research Press
誌名: Biomedical Research
巻: 37
号: 1
開始ページ: 63
終了ページ: 71
出版社 DOI: 10.2220/biomedres.37.63
抄録: To clarify the relationship between ovarian reserve and the developmental competence of bovine oocytes, oocyte-granulosa complexes (OGCs) collected from early antral follicles (≤1 mm) in ovaries with high (≥25) and low (<25) antral follicle counts (AFCs) were used. OGCs derived from different AFC groups were cultured for growth followed by maturation, fertilization and blastocyst formation. Viability of OGCs during growth culture was similar between groups; however, OGCs in the high-AFC group had a larger number of granulosa cells than the low-AFC group at 12 days of growth. The proportion of matured oocytes in the high-AFC group was higher than that in the low-AFC group. Mitochondrial activity of oocytes before maturation in the high-AFC group was higher than that in the low-AFC group; however, accumulation of reactive oxygen species was similar between groups. Cleavage rate in the high-AFC group tended to be higher than that in the low-AFC group, although blastocyst development was similar between groups. In conclusion, oocytes derived from ovaries with high AFC have higher maturational ability and fertilizability than those from low AFC. The difference may be caused by high proliferation of granulosa cells from ovaries with high AFC.
資料タイプ: article
URI: http://hdl.handle.net/2115/60734
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 永野 昌志

 

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