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Uncovering potential of Indonesian medicinal plants on glucose uptake enhancement and lipid suppression in 3T3-L1 adipocytes

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Title: Uncovering potential of Indonesian medicinal plants on glucose uptake enhancement and lipid suppression in 3T3-L1 adipocytes
Authors: Lahrita, Lucy Browse this author
Kato, Eisuke Browse this author →KAKEN DB
Kawabata, Jun Browse this author →KAKEN DB
Keywords: Diabetes
Eurycoma longifolia Jack
Piper nigrum L.
Tropical plants
Issue Date: 20-Jun-2015
Publisher: Elsevier
Journal Title: Journal of Ethnopharmacology
Volume: 168
Start Page: 229
End Page: 236
Publisher DOI: 10.1016/j.jep.2015.03.082
PMID: 25862960
Abstract: Ethnopharmacological relevance : As obesity is a key factor in the development of type 2 diabetes, lowering lipid accumulation in adipose tissues is as important as increasing insulin sensitivity in diabetic patients. The selected plant extracts used in this screen have been traditionally used in Indonesian medicine for the treatment of diabetes and its complications. Aim of the study : To investigate of the selected plants to both increase insulin sensitivity through the enhancement of glucose uptake after insulin induction in adipocytes and suppress lipid production in the same target cells. Materials and methods : Dried Indonesian medicinal plants were extracted with 50% (v/v) aq. Methanol. The extracts were dissolved in 50% DMSO when tested on 3T3-L1 adipocytes. The screening platform consists of insulin-induced glucose uptake, lipid accumulation, and cell viability. Initially, an enzymatic fluorescence assay was designed to demonstrate the enhancement of 2-deoxyglucose (2-DG) uptake after insulin induction. Different concentrations of the extracts that enhanced glucose uptake were subjected to lipid accumulation assay using Oil Red O staining. Potential extracts based on lipid suppression were subsequently assessed by CCK-8 cell viability assay to distinguish lipid reduction activity from cytotoxicity. Results : Out of 59 plants, 13 plants demonstrated their ability to increase glucose uptake in 3T3-L1 adipocytes after insulin induction, and 4 of these plants’ extracts suppressed lipid production of the cells. The CCK-8 assay results of those 4 plant extracts suggest that the inhibition activity of Eurycoma longifolia Jack (root) and Piper nigrum L.(fruits) extracts is not attributed to their cytotoxicity in the adipose cells. Both of the plant extracts increased glucose uptake by more than 200% at 50 g/mL and suppressed lipid accumulation in a concentration-dependent manner. Conclusions : Screening of selected Indonesian medicinal plants has uncovered the potentials of Eurycoma longifolia Jack (root) and Piper nigrum L. (fruits) with dual active functions, increasing insulin sensitivity through the enhancement of glucose uptake and reducing lipid accumulation in adipose cells. These findings suggest that the ability of both plants to suppress lipid production would provide additional benefits in the treatment of diabetes.
Rights: © 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 加藤 英介

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