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Hokkaido University Collection of Scholarly and Academic Papers >
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Cloning and Characterization of a Novel Chondroitin Sulfate/Dermatan Sulfate 4-O-Endosulfatase from a Marine Bacterium
Title: | Cloning and Characterization of a Novel Chondroitin Sulfate/Dermatan Sulfate 4-O-Endosulfatase from a Marine Bacterium |
Authors: | Wang, Wenshuang Browse this author | Han, Wenjun Browse this author | Cai, Xingya Browse this author | Zheng, Xiaoyu Browse this author | Sugahara, Kazuyuki Browse this author →KAKEN DB | Li, Fuchuan Browse this author |
Keywords: | Chondroitin Sulfate | Dermatan Sulfate | Glycosaminoglycan | Oligosaccharide | Polysaccharide | Endosulfatase | Marine Bacterium | Sulfated Polysaccharide |
Issue Date: | 20-Mar-2015 |
Publisher: | American Society for Biochemistry and Molecular Biology (ASBMB) |
Journal Title: | Journal of Biological Chemistry |
Volume: | 290 |
Issue: | 12 |
Start Page: | 7823 |
End Page: | 7832 |
Publisher DOI: | 10.1074/jbc.M114.629154 |
PMID: | 25648894 |
Abstract: | Sulfatases are potentially useful tools for structure-function studies of glycosaminoglycans (GAGs). To date, various GAG exosulfatases have been identified in eukaryotes and prokaryotes. However, endosulfatases that act on GAGs have rarely been reported. Recently, a novel HA and CS lyase (HCLase) was identified for the first time from a marine bacterium (Han, W., Wang, W., Zhao, M., Sugahara, K., and Li, F. (2014) J. Biol. Chem. 289, 27886–27898). In this study, a putative sulfatase gene, closely linked to the hclase gene in the genome, was recombinantly expressed and characterized in detail. The recombinant protein showed a specific N-acetylgalactosamine-4-O-sulfatase activity that removes 4-O-sulfate from both disaccharides and polysaccharides of chondroitin sulfate (CS)/dermatan sulfate (DS), suggesting that this sulfatase represents a novel endosulfatase. The novel endosulfatase exhibited maximal reaction rate in a phosphate buffer (pH 8.0) at 30 °C and effectively removed 17–65% of 4-O-sulfates from various CS and DS and thus significantly inhibited the interactions of CS and DS with a positively supercharged fluorescent protein. Moreover, this endosulfatase significantly promoted the digestion of CS by HCLase, suggesting that it enhances the digestion of CS/DS by the bacterium. Therefore, this endosulfatase is a potential tool for use in CS/DS-related studies and applications. |
Rights: | This research was originally published in Journal of Biological Chemistry. Wenshuang Wang, Wenjun Han, Xingya Cai, Xiaoyu Zheng, Kazuyuki Sugahara and Fuchuan Li. Cloning and Characterization of a Novel Chondroitin Sulfate/Dermatan Sulfate 4-O-Endosulfatase from a Marine Bacterium. Journal of Biological Chemistry. 2015; Vol:290(12) p.7823-7832 © the American Society for Biochemistry and Molecular Biology |
Type: | article |
URI: | http://hdl.handle.net/2115/62913 |
Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 菅原 一幸
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