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Characterization of a heat-activated retrotransposon in natural accessions of Arabidopsis thaliana

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Title: Characterization of a heat-activated retrotransposon in natural accessions of Arabidopsis thaliana
Authors: Masuda, Seiji Browse this author
Nozawa, Kosuke Browse this author
Matsunaga, Wataru Browse this author
Masuta, Yukari Browse this author
Kawabe, Akira Browse this author
Kato, Atsushi Browse this author →KAKEN DB
Ito, Hidetaka Browse this author →KAKEN DB
Keywords: Arabidopsis
heat stress
mutation
ONSEN
retrotransposon
Issue Date: Dec-2016
Publisher: 日本遺伝学会
Journal Title: Genes & Genetic Systems
Volume: 91
Issue: 6
Start Page: 293
End Page: 299
Publisher DOI: 10.1266/ggs.16-00045
PMID: 27980240
Abstract: Natural accessions are used for studying intraspecies genetic variation in the model plant Arabidopsis thaliana in order to address fundamental questions of evolution. Transposable elements are responsible for a wide range of mutations and play significant roles in shaping a genome over evolutionary time. In the present study, we aimed to characterize ONSEN, a heat-activated long terminal repeat (LTR) retrotransposon, in natural A. thaliana accessions. Southern blot analysis demonstrated that ONSEN was present in all the studied accessions, but the copy number was diverse. Olympia-1 contained a single ONSEN copy, located in the centromere of Chromosome 3. The stop codons in Olympia-1 ONSEN presumably abolish integrase activity, which in turn presumably renders the retrotransposon non-functional. Hybridization of Col-0 with Olympia-1 showed that several ONSEN copies in Col-0 were activated by heat stress and maintained their transpositional activity in the progeny.
Type: article
URI: http://hdl.handle.net/2115/65346
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 伊藤 秀臣

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