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Evolution of corticosteroid specificity for human, chicken, alligator and frog glucocorticoid receptors

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Title: Evolution of corticosteroid specificity for human, chicken, alligator and frog glucocorticoid receptors
Authors: Katsu, Yoshinao Browse this author →KAKEN DB
Kohno, Satomi Browse this author
Oka, Kaori Browse this author
Baker, Michael E. Browse this author
Keywords: Glucocorticoid receptor
Glucocorticoid evolution
Allosteric regulation
Alligator
Xenopus
Chicken
Issue Date: Sep-2016
Publisher: Elsevier
Journal Title: Steroids
Volume: 113
Start Page: 38
End Page: 45
Publisher DOI: 10.1016/j.steroids.2016.06.005
PMID: 27317937
Abstract: We investigated the evolution of the response of human, chicken, alligator and frog glucocorticoid receptors (GRs) to dexamethasone, cortisol, cortisone, corticosterone, 11-deoxycorticosterone, 11-deoxycortisol and aldosterone. We find significant differences among these vertebrates in the transcriptional activation of their full length GRs by these steroids, indicating that there were changes in the specificity of the GR for steroids during the evolution of terrestrial vertebrates. To begin to study the role of interactions between different domains on the GR in steroid sensitivity and specificity for terrestrial GRs, we investigated transcriptional activation of truncated GRs containing their hinge domain and ligand binding domain (LBD) fused to a GAL4 DNA binding domain (GAL4-DBD). Compared to corresponding full length GRs, transcriptional activation of GAL4-DBD_GR-hinge/LBD constructs required higher steroid concentrations and displayed altered steroid specificity, indicating that interactions between the hinge/LBD and other domains are important in glucocorticoid activation of these terrestrial GRs. (C) 2016 Elsevier Inc. All rights reserved.
Rights: © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/67084
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 勝 義直

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