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Degenerative and regenerative features of myofibers differ among skeletal muscles in a murine model of muscular dystrophy

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J Muscle Res Cell Motil v.37(4-5) p.153-164.pdf1.39 MBPDF見る/開く
この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/67282

タイトル: Degenerative and regenerative features of myofibers differ among skeletal muscles in a murine model of muscular dystrophy
著者: Ikeda, Teppei 著作を一覧する
Ichii, Osamu 著作を一覧する
Otsuka-Kanazawa, Saori 著作を一覧する
Nakamura, Teppei 著作を一覧する
Elewa, Yaser Hosny Ali 著作を一覧する
Kon, Yasuhiro 著作を一覧する
キーワード: Muscular dystrophy
mdx mouse
Skeletal muscle remodeling
Inflammation
Triceps brachii
Costal part of the diaphragm
発行日: 2016年10月
出版者: Springer
誌名: Journal of Muscle Research and Cell Motility
巻: 37
号: 4-5
開始ページ: 153
終了ページ: 164
出版社 DOI: 10.1007/s10974-016-9452-6
抄録: Skeletal muscle myofibers constantly undergo degeneration and regeneration. Histopathological features of 6 skeletal muscles (cranial tibial [CT], gastrocnemius, quadriceps femoris, triceps brachii [TB], lumbar longissimus muscles, and costal part of the diaphragm [CPD]) were compared using C57BL/10ScSn-Dmd (mdx) (mdx) mice, a model for muscular dystrophy versus control, C57BL/10 mice. Body weight and skeletal muscle mass were lower in mdx mice than the control at 4 weeks of age; these results were similar at 6-30 weeks. Additionally, muscular lesions were observed in all examined skeletal muscles in mdx mice after 4 weeks, but none were noted in the controls. Immunohistochemical staining revealed numerous paired box 7-positive satellite cells surrounding the embryonic myosin heavy chain-positive regenerating myofibers, while the number of the former and staining intensity of the latter decreased as myofiber regeneration progressed. Persistent muscular lesions were observed in skeletal muscles of mdx mice between 4 and 14 weeks of age, and normal myofibers decreased with age. Number of muscular lesions was lowest in CPD at all ages examined, while the ratio of normal myofibers was lowest in TB at 6 weeks. In CT, TB, and CPD, Iba1-positive macrophages, the main inflammatory cells in skeletal muscle lesions, showed a significant positive correlation with the appearance of regenerating myofibers. Additionally, B220-positive B-cells showed positive and negative correlation with regenerating and regenerated myofibers, respectively. Our data suggest that degenerative and regenerative features of myofibers differ among skeletal muscles and that inflammatory cells are strongly associated with regenerative features of myofibers in mdx mice.
Rights: The final publication is available at link.springer.com
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/67282
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 昆 泰寛

 

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