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Degenerative and regenerative features of myofibers differ among skeletal muscles in a murine model of muscular dystrophy

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J Muscle Res Cell Motil v.37(4-5) p.153-164.pdf1.39 MBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/67282

Title: Degenerative and regenerative features of myofibers differ among skeletal muscles in a murine model of muscular dystrophy
Authors: Ikeda, Teppei Browse this author
Ichii, Osamu Browse this author →KAKEN DB
Otsuka-Kanazawa, Saori Browse this author →KAKEN DB
Nakamura, Teppei Browse this author
Elewa, Yaser Hosny Ali Browse this author →ORCID
Kon, Yasuhiro Browse this author →KAKEN DB
Keywords: Muscular dystrophy
mdx mouse
Skeletal muscle remodeling
Inflammation
Triceps brachii
Costal part of the diaphragm
Issue Date: Oct-2016
Publisher: Springer
Journal Title: Journal of Muscle Research and Cell Motility
Volume: 37
Issue: 4-5
Start Page: 153
End Page: 164
Publisher DOI: 10.1007/s10974-016-9452-6
PMID: 27472930
Abstract: Skeletal muscle myofibers constantly undergo degeneration and regeneration. Histopathological features of 6 skeletal muscles (cranial tibial [CT], gastrocnemius, quadriceps femoris, triceps brachii [TB], lumbar longissimus muscles, and costal part of the diaphragm [CPD]) were compared using C57BL/10ScSn-Dmd (mdx) (mdx) mice, a model for muscular dystrophy versus control, C57BL/10 mice. Body weight and skeletal muscle mass were lower in mdx mice than the control at 4 weeks of age; these results were similar at 6-30 weeks. Additionally, muscular lesions were observed in all examined skeletal muscles in mdx mice after 4 weeks, but none were noted in the controls. Immunohistochemical staining revealed numerous paired box 7-positive satellite cells surrounding the embryonic myosin heavy chain-positive regenerating myofibers, while the number of the former and staining intensity of the latter decreased as myofiber regeneration progressed. Persistent muscular lesions were observed in skeletal muscles of mdx mice between 4 and 14 weeks of age, and normal myofibers decreased with age. Number of muscular lesions was lowest in CPD at all ages examined, while the ratio of normal myofibers was lowest in TB at 6 weeks. In CT, TB, and CPD, Iba1-positive macrophages, the main inflammatory cells in skeletal muscle lesions, showed a significant positive correlation with the appearance of regenerating myofibers. Additionally, B220-positive B-cells showed positive and negative correlation with regenerating and regenerated myofibers, respectively. Our data suggest that degenerative and regenerative features of myofibers differ among skeletal muscles and that inflammatory cells are strongly associated with regenerative features of myofibers in mdx mice.
Rights: The final publication is available at link.springer.com
Type: article (author version)
URI: http://hdl.handle.net/2115/67282
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 昆 泰寛

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