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Assessment of antimicrobial and biocompatible effects ofAu25(Capt)18 clusters photoexcited by blue LED
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| Title: | Assessment of antimicrobial and biocompatible effects ofAu25(Capt)18 clusters photoexcited by blue LED |
| Other Titles: | カプトプリル保護金クラスターの青色LED光照射による抗菌効果と生体親和性の評価 |
| Authors: | 宮田, さほり Browse this author |
| Issue Date: | 23-Mar-2017 |
| Publisher: | Hokkaido University |
| Abstract: | Antimicrobial photodynamic therapy (aPDT) has beneficial effects in dental treatment. We applied captopril-protected gold (Au25(Capt)18) clusters as a novel biosafe photosensitizer for aPDT. Photoexcited Au25(Capt)18 clusters under dental blue light emitting diode (LED) irradiation generated a singlet oxygen (1O2). Accordingly, the antimicrobial and cytotoxic effects of Au25(Capt)18 clusters under blue LED irradiation were evaluated. Successful application of photoexcited Au25(Capt)18 clusters to aPDT was demonstrated by dose-dependent decrease in the turbidity of oral bacterial cells, such as Streptococcus mutans, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. In addition, blue LED use alone slightly diminished the turbidity of bacterial cells. Morphological observation revealed that application of clusters stimulated destruction of bacterial cell walls. However, photoexcited Au clusters did not negatively affect the adhesion, spreading and proliferation of NIH3T3 and MC3T3-E1 cells, in particular, at lower dose. In comparative assessments of Au clusters and methylene blue, Au25(Capt)18 clusters had higher biocompatibility than methylene blue. We found that a combination of Au25(Capt)18 clusters and blue LED irradiation exhibited good antimicrobial and biosafe characteristics, which would be desirable for aPDT in dental use. |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第12603号 |
| Degree Level: | 博士 |
| Degree Discipline: | 歯学 |
| Examination Committee Members: | (主査) 教授 柴田 健一郎, 准教授 菅谷 勉, 教授 吉田 靖弘 |
| Degree Affiliation: | 歯学研究科（口腔医学専攻） |
| Type: | theses (doctoral) |
| URI: | http://hdl.handle.net/2115/67339 |
| Appears in Collections: | 学位論文 (Theses) > 博士 （歯学）
課程博士 (Doctorate by way of Advanced Course) > 歯学院(Graduate School of Dental Medicine)
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