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A novel reverse genetics system for production of infectious West Nile virus using homologous recombination in mammalian cells
Title: | A novel reverse genetics system for production of infectious West Nile virus using homologous recombination in mammalian cells |
Authors: | Kobayashi, Shintaro Browse this author →KAKEN DB | Yoshii, Kentaro Browse this author →KAKEN DB | Hirano, Minato Browse this author | Muto, Memi Browse this author | Kariwa, Hiroaki Browse this author →KAKEN DB |
Keywords: | Reverse genetics | West Nile virus | Homologous recombination |
Issue Date: | 16-Nov-2016 |
Publisher: | Elsevier |
Journal Title: | Journal of virological methods |
Volume: | 240 |
Start Page: | 14 |
End Page: | 20 |
Publisher DOI: | 10.1016/j.jviromet.2016.11.006 |
PMID: | 27865748 |
Abstract: | Reverse genetics systems facilitate investigation of many aspects of the life cycle and pathogenesis of viruses. However, genetic instability in Escherichia coli has hampered development of a reverse genetics system for West Nile virus (WNV). In this study, we developed a novel reverse genetics system for WNV based on homologous recombination in mammalian cells. Introduction of the DNA fragment coding for the WNV structural protein together with a DNA-based replicon resulted in the release of infectious WNV. The growth rate and plague size of the recombinant virus were almost identical to those of the parent WNV. Furthermore, chimeric WNV was produced by introducing the DNA fragment coding for the structural protein and replicon plasmid derived from various strains. Here, we report development of a novel system that will facilitate research into WNV infection. (C) 2016 Elsevier B.V. All rights reserved. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/67606 |
Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 小林 進太郎
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