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A novel reverse genetics system for production of infectious West Nile virus using homologous recombination in mammalian cells

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/67606

Title: A novel reverse genetics system for production of infectious West Nile virus using homologous recombination in mammalian cells
Authors: Kobayashi, Shintaro Browse this author →KAKEN DB
Yoshii, Kentaro Browse this author →KAKEN DB
Hirano, Minato Browse this author
Muto, Memi Browse this author
Kariwa, Hiroaki Browse this author →KAKEN DB
Keywords: Reverse genetics
West Nile virus
Homologous recombination
Issue Date: 16-Nov-2016
Publisher: Elsevier
Journal Title: Journal of virological methods
Volume: 240
Start Page: 14
End Page: 20
Publisher DOI: 10.1016/j.jviromet.2016.11.006
PMID: 27865748
Abstract: Reverse genetics systems facilitate investigation of many aspects of the life cycle and pathogenesis of viruses. However, genetic instability in Escherichia coli has hampered development of a reverse genetics system for West Nile virus (WNV). In this study, we developed a novel reverse genetics system for WNV based on homologous recombination in mammalian cells. Introduction of the DNA fragment coding for the WNV structural protein together with a DNA-based replicon resulted in the release of infectious WNV. The growth rate and plague size of the recombinant virus were almost identical to those of the parent WNV. Furthermore, chimeric WNV was produced by introducing the DNA fragment coding for the structural protein and replicon plasmid derived from various strains. Here, we report development of a novel system that will facilitate research into WNV infection. (C) 2016 Elsevier B.V. All rights reserved.
Type: article (author version)
URI: http://hdl.handle.net/2115/67606
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小林 進太郎

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