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Isolation and molecular characterization of extended-spectrum β-lactamase producing Escherichia coli from industrial food animals in Mekong Delta, Vietnam

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Title: Isolation and molecular characterization of extended-spectrum β-lactamase producing Escherichia coli from industrial food animals in Mekong Delta, Vietnam
Authors: Hinenoya, Atsushi Browse this author
Tran, Suong Thi Thu Browse this author
Nguyen, Ngu Trong Browse this author
Nguyen, Ha Cong Browse this author
Nguyen, Doan Duy Le Browse this author
Hoang, Phuong Hoai Browse this author
Awasthi, Sharda Prasad Browse this author
Hassan, Jayedul Browse this author
Sumimura, Yoshinori Browse this author
Yamamoto, Yoshimasa Browse this author
Yamasaki, Shinji Browse this author
Keywords: antimicrobials
Escherichia coli
Issue Date: Feb-2018
Journal Title: Japanese Journal of Veterinary Research
Volume: 66
Issue: 1
Start Page: 1
End Page: 12
Abstract: The aim of this study was to investigate if extended-spectrum β-lactamase producing Escherichia coli (ESBL-EC) is present in intestinal tracts of apparently healthy broiler chickens at large-scale chicken farm and pigs, and their environments in Vietnam. ESBL-EC was isolated from 86.7% cloacal swabs of chickens (13 out of 15), 55.0% rectal swabs of pigs (11 out of 20) and 100% from their surroundings (2 beddings in a chicken farm and 2 drainages in pig farms). All the isolates from chicken and pig farms were multidrug-resistant. Interestingly, 94.7% (36/38) isolates from chicken were resistant to ciprofloxacin and mcr-1 gene-positive (related to colistin resistance), respectively, while ciprofloxacin resistance and mcr-1 gene was found in only 12.8% (5/39) and none (0/39) from pig, respectively. CTX-M type in most of the chicken isolates belonged to group-1 whereas that in the pig isolates belonged to group-9. Virulence gene profiling revealed that some of these isolates indeed carry eae or astA pathogenic genes. Plasmid profiling and PFGE analysis indicated that most of them showed various genotypes although some isolates showed nearly identical genotype, suggesting that a number of ESBL-EC with various genotypes were distributed in chickens and pigs in Mekong Delta. To the best of our knowledge, this is the first report regarding isolation of ESBL-EC from broiler chickens in large scale-farms and pigs in Vietnam. Taken together, these results suggest that chickens and pigs in Mekong Delta, Vietnam used for food industry could also serve as reservoirs of ESBL-EC isolates carrying virulence genes.
Type: bulletin (article)
Appears in Collections:Japanese Journal of Veterinary Research > Volume 66 Number 1

Submitter: 獣医学部図書室

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