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Volume 66 Number 3 >

A clinical case of enzootic bovine leukosis in a 13-month-old Holstein heifer

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Title: A clinical case of enzootic bovine leukosis in a 13-month-old Holstein heifer
Authors: Maezawa, Masaki Browse this author
Watanabe, Ken-ichi Browse this author
Horiuchi, Noriyuki Browse this author
Matsumoto, Kotaro Browse this author
Kobayashi, Yoshiyasu Browse this author
Inokuma, Hisashi Browse this author
Keywords: heifer
enzootic bovine leukosis
inverse PCR
Issue Date: Aug-2018
Publisher: Faculty of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 66
Issue: 3
Start Page: 209
End Page: 213
Abstract: A 13-month-old Holstein heifer presented with emaciation and enlarged superficial lymph nodes. Hematological examination showed remarkable lymphocytosis with atypical lymphocytes. Increased activities of serum total lactate dehydrogenase and thymidine kinase were detected. Fine needle aspiration cytology of the subiliac lymph node revealed large lymphoblasts undergoing mitosis. Nested PCR for bovine leukemia virus (BLV) and antibody against BLV were both positive. BLV proviral load was relatively high. Pathological examination confirmed that the case was diffuse large B-cell lymphoma. B-cell monoclonality was also confirmed by PCR for V-D-J junctional variability. Monoclonal integration of BLV provirus in the bovine genome was demonstrated with the inverse PCR method. The heifer was definitively diagnosed with enzootic bovine leukosis based on these findings.
Type: bulletin (article)
Appears in Collections:Japanese Journal of Veterinary Research > Volume 66 Number 3

Submitter: 獣医学部図書室

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