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Localization of major yolk protein in the digestive tract of the sea urchin Strongylocentrotus intermedius

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/71422

Title: Localization of major yolk protein in the digestive tract of the sea urchin Strongylocentrotus intermedius
Authors: Okashita, Yuki Browse this author
Wang, Heng Browse this author
Tsue, Shiori Browse this author
Nishimiya, Osamu Browse this author
Ura, Kazuhiro Browse this author →KAKEN DB
Takagi, Yasuaki Browse this author →KAKEN DB
Keywords: Sea urchin
Major yolk protein
Immunohistochemistry
In situ hybridization
Intestine
Issue Date: Sep-2017
Publisher: Springer
Journal Title: Fisheries science
Volume: 83
Issue: 5
Start Page: 803
End Page: 810
Publisher DOI: 10.1007/s12562-017-1118-9
Abstract: In the present study, we examined the localization of the major yolk protein (MYP) in the intestine of the sea urchin Strongylocentrotus intermedius. First, partial MYP complementary DNA was isolated from the sea urchin intestine. The expression level of MYP messenger RNA (mRNA) along the sea urchin digestive tract is highest in the intestine, so we performed in situ hybridization and immunohistochemical analysis using this tissue. No MYP mRNA was detected in the luminal epithelium, connective tissue, muscle tissue, or coelomic epithelium by in situ hybridization analysis. Positive immunohistochemical staining was observed in the luminal epithelium, inner epithelium and connective tissue, the signal being strongest in the latter. We conclude that MYP synthesized in the inner epithelial cells is moved to and stored in connective tissue and the luminal epithelium, before being secreted into the body cavity and the inner digestive cavity of the sea urchin.
Rights: The final publication is available at www.springerlink.com via http://dx.doi.org/10.1007/s12562-017-1118-9.
Type: article (author version)
URI: http://hdl.handle.net/2115/71422
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 浦 和寛

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