Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Localization of major yolk protein in the digestive tract of the sea urchin Strongylocentrotus intermedius
Title: | Localization of major yolk protein in the digestive tract of the sea urchin Strongylocentrotus intermedius |
Authors: | Okashita, Yuki Browse this author | Wang, Heng Browse this author | Tsue, Shiori Browse this author | Nishimiya, Osamu Browse this author | Ura, Kazuhiro Browse this author →KAKEN DB | Takagi, Yasuaki Browse this author →KAKEN DB |
Keywords: | Sea urchin | Major yolk protein | Immunohistochemistry | In situ hybridization | Intestine |
Issue Date: | Sep-2017 |
Publisher: | Springer |
Journal Title: | Fisheries science |
Volume: | 83 |
Issue: | 5 |
Start Page: | 803 |
End Page: | 810 |
Publisher DOI: | 10.1007/s12562-017-1118-9 |
Abstract: | In the present study, we examined the localization of the major yolk protein (MYP) in the intestine of the sea urchin Strongylocentrotus intermedius. First, partial MYP complementary DNA was isolated from the sea urchin intestine. The expression level of MYP messenger RNA (mRNA) along the sea urchin digestive tract is highest in the intestine, so we performed in situ hybridization and immunohistochemical analysis using this tissue. No MYP mRNA was detected in the luminal epithelium, connective tissue, muscle tissue, or coelomic epithelium by in situ hybridization analysis. Positive immunohistochemical staining was observed in the luminal epithelium, inner epithelium and connective tissue, the signal being strongest in the latter. We conclude that MYP synthesized in the inner epithelial cells is moved to and stored in connective tissue and the luminal epithelium, before being secreted into the body cavity and the inner digestive cavity of the sea urchin. |
Rights: | The final publication is available at www.springerlink.com via http://dx.doi.org/10.1007/s12562-017-1118-9. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/71422 |
Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 浦 和寛
|