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Sub-Zeptomole Detection in a Microfabricated Glass Channel by Thermal-Lens Microscopy.

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Title: Sub-Zeptomole Detection in a Microfabricated Glass Channel by Thermal-Lens Microscopy.
Authors: SATO, Kiyoshi Browse this author
KAWANISHI, Hiroaki Browse this author
TOKESHI, Manabu Browse this author →KAKEN DB
KITAMORI, Takehiko Browse this author
SAWADA, Tsuguo Browse this author
Keywords: Thermal-lens microscope
integrated chemistry laboratory convection
Issue Date: Jun-1999
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 15
Issue: 6
Start Page: 525
End Page: 529
Publisher DOI: 10.2116/analsci.15.525
Abstract: A thermal-lens microscope which we developed was applied to an ultramicro quantity determination of a dye in an aque-ous solution filling a microchannel (150 µm wide and 100 µm deep) fabricated in a quartz glass substrate. The detection volume, which corresponded to the confocal volume, was estimated to be 1.3 fl. The detection limit of the dye molecules was 160 ymol, and the calibration line showed good linearity in the sub-zmol-to-zmol region. This detection sensitivity is equivalent to that of laser-induced fluorometry. The thermal-lens signal measured in the microchannel was more stable than that measured in a liquid micro space between a slide glass and a cover glass, which was much wider than the microchannel. This may have been due to a suppression of convection in the microchannel. The thermal lens method can be applied to non-fluorescent chemical species, and is thus very suitable detection method for integrated chemistry sys-tems.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次 学

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