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Aqueous Micellar Two-Phase Systems for Protein Separation

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Title: Aqueous Micellar Two-Phase Systems for Protein Separation
Authors: TANI, Hirofumi Browse this author →KAKEN DB
KAMIDATE, Tamio Browse this author
WATANABE, Hiroto Browse this author
Keywords: Surfactant
phase separation
cloud point
Issue Date: Oct-1998
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 14
Issue: 5
Start Page: 875
End Page: 888
Publisher DOI: 10.2116/analsci.14.875
Abstract: The extractive technique for protein purification based on two-phase separation in aqueous micellar solutions (aqueous micellar two-phase system (AMTPS)) is reviewed. The micellar solution of a nonionic surfactant, such as polyoxyethyl-ene alkyl ether, which is most frequently used for protein extraction, separates into two phases upon heating above its cloud point. The two phases consist of a surfactant-depleted phase (aqueous phase) and a surfactant-rich phase. Hydrophilic proteins are partitioned to the aqueous phase and hydrophobic membrane proteins are extracted into the sur-factant-rich phase. Because of the methodological simplicity and rapidity, this technique has become an effective means, and thus has been widely used for the purification and characterization of proteins. In contrast to polyoxyethylene alkyl ether, micellar solutions of a zwitterionic surfactant, such as alkylammoniopropyl sulfate, separate below the critical temperature. Alkylglucosides can also separate into two phases upon adding water-soluble polymers. Recently, these two-phase systems have been exploited for protein separation. Additionally, hydrophobic affinity ligands, charged polymers, and ionic surfactants have been successfully used for controlling the extractability of proteins in AMTPS.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 谷 博文

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