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Polymer-Induced Phase Separation in Aqueous Micellar Solutions of Alkylglucosides for Protein Extraction

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Title: Polymer-Induced Phase Separation in Aqueous Micellar Solutions of Alkylglucosides for Protein Extraction
Authors: SAITOH, Tohru Browse this author
TANI, Hirofumi Browse this author →KAKEN DB
KAMIDATE, Tamio Browse this author
KAMATAKI, Tetsuya Browse this author
WATANABE, Hiroto Browse this author
Keywords: phase separation
protein extraction
Issue Date: Apr-1994
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 10
Issue: 2
Start Page: 299
End Page: 303
Publisher DOI: 10.2116/analsci.10.299
Abstract: Aqueous micellar solutions of alkylglucosides were separated into two phases at 0°C upon addition of polyethylene glycol 6000 (PEG) or Dextran T-500 (Dextran). One was an aqueous phase in which hydrophilic proteins, cytochrome c and peroxidase (horseradish), were retained, and the other was a surfactant-rich phase into which hydrophobic membrane proteins, bacteriorhodopsin and cytochrome b5, were extracted. A combination of octyl-β-D-thioglucoside (OTG) (or nonyl-β-D-glucoside (NG)) with PEG (or Dextran) was the best choice for extraction of hydrophobic proteins. Extraction yields (50-90%) and concentration factors (7-30) of the hydrophobic proteins were dependent on the types of nonionic surfactants and water-soluble polymers. Solubilization and phase separation in processing cell membranes could be made in a single step at 0°C. Hence the present method would be useful for the purification of thermolabile proteins.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 谷 博文

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