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Polymer-Induced Phase Separation in Aqueous Micellar Solutions of Alkylglucosides for Protein Extraction
| Title: | Polymer-Induced Phase Separation in Aqueous Micellar Solutions of Alkylglucosides for Protein Extraction |
| Authors: | SAITOH, Tohru Browse this author | | TANI, Hirofumi Browse this author →KAKEN DB | | KAMIDATE, Tamio Browse this author | | KAMATAKI, Tetsuya Browse this author | | WATANABE, Hiroto Browse this author |
| Keywords: | phase separation | | alkylglucoside | | polymer | | protein extraction |
| Issue Date: | Apr-1994 |
| Publisher: | 日本分析化学会 |
| Journal Title: | Analytical Sciences |
| Volume: | 10 |
| Issue: | 2 |
| Start Page: | 299 |
| End Page: | 303 |
| Publisher DOI: | 10.2116/analsci.10.299 |
| Abstract: | Aqueous micellar solutions of alkylglucosides were separated into two phases at 0°C upon addition of polyethylene glycol 6000 (PEG) or Dextran T-500 (Dextran). One was an aqueous phase in which hydrophilic proteins, cytochrome c and peroxidase (horseradish), were retained, and the other was a surfactant-rich phase into which hydrophobic membrane proteins, bacteriorhodopsin and cytochrome b5, were extracted. A combination of octyl-β-D-thioglucoside (OTG) (or nonyl-β-D-glucoside (NG)) with PEG (or Dextran) was the best choice for extraction of hydrophobic proteins. Extraction yields (50-90%) and concentration factors (7-30) of the hydrophobic proteins were dependent on the types of nonionic surfactants and water-soluble polymers. Solubilization and phase separation in processing cell membranes could be made in a single step at 0°C. Hence the present method would be useful for the purification of thermolabile proteins. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71709 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 谷 博文
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