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Microfluidic Approaches for Protein Crystal Structure Analysis

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Title: Microfluidic Approaches for Protein Crystal Structure Analysis
Authors: MAEKI, Masatoshi Browse this author →KAKEN DB
YAMAGUCHI, Hiroshi Browse this author
TOKESHI, Manabu Browse this author
MIYAZAKI, Masaya Browse this author
Keywords: microfluidic device
protein crystallization
protein crystallography
in-situ X-ray analysis
Issue Date: 10-Jan-2016
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 32
Issue: 1
Start Page: 3
End Page: 9
Publisher DOI: 10.2116/analsci.32.3
PMID: 26753699
Abstract: This review summarizes two microfluidic-based protein crystallization methods, protein crystallization behavior in the microfluidic devices, and their applications for X-ray crystal structure analysis. Microfluidic devices provide many advantages for protein crystallography; they require small sample volumes, provide high-throughput screening, and allow control of the protein crystallization. A droplet-based protein crystallization method is a useful technique for high-throughput screening and the formation of a single crystal without any complicated device fabrication process. Well-based microfluidic platforms also enable effective protein crystallization. This review also summarizes the protein crystal growth behavior in microfluidic devices as, is known from viewpoints of theoretical and experimental approaches. Finally, we introduce applications of microfluidic devices for on-chip crystal structure analysis.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 真栄城 正寿

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