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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >
Microfluidic Approaches for Protein Crystal Structure Analysis
| Title: | Microfluidic Approaches for Protein Crystal Structure Analysis |
| Authors: | MAEKI, Masatoshi Browse this author →KAKEN DB | | YAMAGUCHI, Hiroshi Browse this author | | TOKESHI, Manabu Browse this author | | MIYAZAKI, Masaya Browse this author |
| Keywords: | microfluidic device | | protein crystallization | | protein crystallography | | in-situ X-ray analysis |
| Issue Date: | 10-Jan-2016 |
| Publisher: | 日本分析化学会 |
| Journal Title: | Analytical Sciences |
| Volume: | 32 |
| Issue: | 1 |
| Start Page: | 3 |
| End Page: | 9 |
| Publisher DOI: | 10.2116/analsci.32.3 |
| PMID: | 26753699 |
| Abstract: | This review summarizes two microfluidic-based protein crystallization methods, protein crystallization behavior in the microfluidic devices, and their applications for X-ray crystal structure analysis. Microfluidic devices provide many advantages for protein crystallography; they require small sample volumes, provide high-throughput screening, and allow control of the protein crystallization. A droplet-based protein crystallization method is a useful technique for high-throughput screening and the formation of a single crystal without any complicated device fabrication process. Well-based microfluidic platforms also enable effective protein crystallization. This review also summarizes the protein crystal growth behavior in microfluidic devices as, is known from viewpoints of theoretical and experimental approaches. Finally, we introduce applications of microfluidic devices for on-chip crystal structure analysis. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71744 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 真栄城 正寿
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