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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Health Sciences / Faculty of Health Sciences >
Peer-reviewed Journal Articles, etc >
Lipidomic Profiling on Oxidized Phospholipids in Type 2 Diabetes Mellitus Model Zebrafish
| Title: | Lipidomic Profiling on Oxidized Phospholipids in Type 2 Diabetes Mellitus Model Zebrafish |
| Authors: | Chen, Zhen Browse this author | | Zang, Liqing Browse this author | | Wu, Yue Browse this author | | Nakayama, Hiroko Browse this author | | Shimada, Yasuhito Browse this author | | Shrestha, Rojeet Browse this author | | Zhao, Yaoyao Browse this author | | Miura, Yusuke Browse this author | | Chiba, Hitoshi Browse this author →KAKEN DB | | Hui, Shu-Ping Browse this author →KAKEN DB | | Nishimura, Norihiro Browse this author |
| Keywords: | Lipid oxidation | | oxidized phospholipids | | oxidative stress biomarkers | | type 2 diabetes mellitus | | zebrafish | | LC-MS |
| Issue Date: | Oct-2018 |
| Publisher: | 日本分析化学会 |
| Journal Title: | Analytical sciences |
| Volume: | 34 |
| Issue: | 10 |
| Start Page: | 1201 |
| End Page: | 1208 |
| Publisher DOI: | 10.2116/analsci.18P281 |
| PMID: | 30101832 |
| Abstract: | Type 2 diabetes mellitus is a serious metabolic disorder in the world. Oxidative stress, as a key role on the pathogenesis of diabetes, also results in the oxidation of phospholipids. However, studies on phospholipid oxidation in diabetes, especially directly focusing on oxidized and degraded phospholipid species, are quite limited. In this study, phospholipid profiles of diabetic zebrafish plasma were characterized by LC-HRMS and MS/MS, and the total amounts of each lipid class were compared. Furthermore, the key molecular species as biomarkers in distinguishing control and diabetic samples were investigated by orthogonal partial least squares discriminant analysis. Among the identified 114 phospholipid species in total, there were 11 hydroperoxides, 7 aldehydes, and 19 lysophospholipids found significantly elevated along with the increasing blood glucose, which were known as oxidation or degradation products. Furthermore, lysophosphatidylcholine 20:5 and lysophosphatidylcholine 22:6 were assessed as potential biomarkers in diabetic zebrafish. The current work would not only help to gain further insights into diabetes, but also contribute to find new clinical parameters for the screening of the promising antioxidant agents for its therapies. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/72155 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 惠 淑萍
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