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Lipidomic Profiling on Oxidized Phospholipids in Type 2 Diabetes Mellitus Model Zebrafish

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Title: Lipidomic Profiling on Oxidized Phospholipids in Type 2 Diabetes Mellitus Model Zebrafish
Authors: Chen, Zhen Browse this author
Zang, Liqing Browse this author
Wu, Yue Browse this author
Nakayama, Hiroko Browse this author
Shimada, Yasuhito Browse this author
Shrestha, Rojeet Browse this author
Zhao, Yaoyao Browse this author
Miura, Yusuke Browse this author
Chiba, Hitoshi Browse this author →KAKEN DB
Hui, Shu-Ping Browse this author →KAKEN DB
Nishimura, Norihiro Browse this author
Keywords: Lipid oxidation
oxidized phospholipids
oxidative stress biomarkers
type 2 diabetes mellitus
zebrafish
LC-MS
Issue Date: Oct-2018
Publisher: 日本分析化学会
Journal Title: Analytical sciences
Volume: 34
Issue: 10
Start Page: 1201
End Page: 1208
Publisher DOI: 10.2116/analsci.18P281
PMID: 30101832
Abstract: Type 2 diabetes mellitus is a serious metabolic disorder in the world. Oxidative stress, as a key role on the pathogenesis of diabetes, also results in the oxidation of phospholipids. However, studies on phospholipid oxidation in diabetes, especially directly focusing on oxidized and degraded phospholipid species, are quite limited. In this study, phospholipid profiles of diabetic zebrafish plasma were characterized by LC-HRMS and MS/MS, and the total amounts of each lipid class were compared. Furthermore, the key molecular species as biomarkers in distinguishing control and diabetic samples were investigated by orthogonal partial least squares discriminant analysis. Among the identified 114 phospholipid species in total, there were 11 hydroperoxides, 7 aldehydes, and 19 lysophospholipids found significantly elevated along with the increasing blood glucose, which were known as oxidation or degradation products. Furthermore, lysophosphatidylcholine 20:5 and lysophosphatidylcholine 22:6 were assessed as potential biomarkers in diabetic zebrafish. The current work would not only help to gain further insights into diabetes, but also contribute to find new clinical parameters for the screening of the promising antioxidant agents for its therapies.
Type: article
URI: http://hdl.handle.net/2115/72155
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 惠 淑萍

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