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Osteocyte-directed bone demineralization along canaliculi

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Title: Osteocyte-directed bone demineralization along canaliculi
Authors: Nango, N. Browse this author
Kubota, S. Browse this author
Hasegawa, T. Browse this author →KAKEN DB
Yashiro, W. Browse this author
Momose, A. Browse this author
Matsuo, K. Browse this author
Keywords: Demineralization/remineralization
Mineral metabolism
Osteocytic osteolysis
Osteocyte canaliculus
Synchrotron radiation
Talbot-defocus multiscan tomography
Issue Date: Mar-2016
Publisher: Elsevier
Journal Title: Bone
Volume: 84
Start Page: 279
End Page: 288
Publisher DOI: 10.1016/j.bone.2015.12.006
PMID: 26709236
Abstract: The mammalian skeleton stores calcium and phosphate ions in bone matrix. Osteocytes in osteocyte lacunae extend numerous dendrites into canaliculi less than a micron in diameter and which are distributed throughout bone matrix. Although osteoclasts are the primary bone-resorbing cells, osteocytes also reportedly dissolve hydroxyapatite at peri-lacunar bone matrix. However, robust three-dimensional evidence for peri-canalicular bone mineral dissolution has been lacking. Here we applied a previously reported Talbot-defocus multiscan tomography method for synchrotron X-ray microscopy and analyzed the degree of bone mineralization in mouse cortical bone around the lacuno?canalicular network, which is connected both to blood vessels and the peri- and endosteum. We detected cylindrical low mineral density regions spreading around canaliculi derived froma subset of osteocytes. Transmission electron microscopy revealed both intact and demineralized bone matrix around the canaliculus. Peri-canalicular lowmineral density regionswere also observed in osteopetrotic mice lacking osteoclasts, indicating that osteoclasts are dispensable for peri-canalicular demineralization. These data suggest demineralization can occur from within bone through the canalicular system, and that peri-canalicular demineralization occurs not uniformly but directed by individual osteocytes. Blockade of peri-canalicular demineralization may be a therapeutic strategy to increase bone mass and quality.
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷川 智香

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