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Purification by p-aminobenzoic acid (PABA)-affinity chromatography and the functional reconstitution of the nateglinide/H+ cotransport system in the rat intestinal brush-border membrane

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Title: Purification by p-aminobenzoic acid (PABA)-affinity chromatography and the functional reconstitution of the nateglinide/H+ cotransport system in the rat intestinal brush-border membrane
Authors: Saito, Yoshitaka Browse this author
Itagaki, Shirou Browse this author
Kubo, Sayaka Browse this author
Kobayashi, Masaki Browse this author →KAKEN DB
Hirano, Takeshi Browse this author
Iseki, Ken6 Browse this author →KAKEN DB
Authors(alt): 井関, 健6
Keywords: PEPT
PABA
BBMV
Transporter
Nateglinide
Ceftibuten
Fluorescein
Issue Date: 17-Feb-2006
Publisher: Elsevier Inc
Journal Title: Biochemical and Biophysical Research Communications
Volume: 340
Issue: 3
Start Page: 879
End Page: 886
Publisher DOI: 10.1016/j.bbrc.2005.12.092
PMID: 16403453
Abstract: (−)-N-(trans-4-Isopropylcyclohexanecarbonyl)-d-phenylalanine (nateglinide) is a novel oral hypoglycemic agent possessing a peptide-type bond and a carboxyl group in its structure. Recently, we have shown that nateglinide transport occurs via the ceftibuten/H+ cotransport system, which is distinct from PepT1, and that the fluorescein/H+ cotransport system is involved in the uptake of nateglinide. The aim of this study was to characterize the functional properties of the intestinal nateglinide transporter. In the first part of this study, we demonstrated that the ceftibuten/H+ cotransport system is identical to the fluorescein/H+ cotransport system. We succeeded in purification of the nateglinide transporter from brush-border membranes of the rat small intestine using p-aminobenzoic acid (PABA)-affinity chromatography. We then investigated the functional properties of the nateglinide transporter using proteoliposomes prepared from the PABA-affinity chromatography elute. We demonstrated that nateglinide, ceftibuten, and fluorescein are transported by the same transporter in the intestine.
Relation: http://www.sciencedirect.com/science/journal/0006291X
Type: article (author version)
URI: http://hdl.handle.net/2115/7385
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 井関 健

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