Title: | Fluorescent immunochromatography for rapid and sensitive typing of seasonal influenza viruses |
Authors: | Sakurai, Akira Browse this author |
Takayama, Katsuyoshi Browse this author |
Nomura, Namiko Browse this author →KAKEN DB |
Kajiwara, Naoki Browse this author →KAKEN DB |
Okamatsu, Masatoshi Browse this author →KAKEN DB |
Yamamoto, Naoki Browse this author |
Tamura, Tsuruki Browse this author |
Yamada, Jitsuho Browse this author |
Hashimoto, Masako Browse this author |
Sakoda, Yoshihiro Browse this author →KAKEN DB |
Suda, Yoshihiko Browse this author |
Kobayashi, Yukuharu Browse this author |
Kida, Hiroshi Browse this author →KAKEN DB |
Shibasaki, Futoshi Browse this author →KAKEN DB |
Issue Date: | 4-Feb-2015 |
Publisher: | Public Library of Science |
Journal Title: | PLoS One |
Volume: | 10 |
Issue: | 2 |
Start Page: | e0116715 |
Publisher DOI: | 10.1371/journal.pone.0116715 |
PMID: | 25650570 |
Abstract: | Lateral flow tests also known as Immunochromatography (IC) is an antigen-detection method conducted on a nitrocellulose membrane that can be completed in less than 20 min. IC has been used as an important rapid test for clinical diagnosis and surveillance of influenza viruses, but the IC sensitivity is relatively low (approximately 60%) and the limit of detection (LOD) is as low as 10³ pfu per reaction. Recently, we reported an improved IC assay using antibodies conjugated with fluorescent beads (fluorescent immunochromatography; FLIC) for subtyping H5 influenza viruses (FLIC-H5). Although the FLIC strip must be scanned using a fluorescent reader, the sensitivity (LOD) is significantly improved over that of conventional IC methods. In addition, the antibodies which are specific against the subtypes of influenza viruses cannot be available for the detection of other subtypes when the major antigenicity will be changed. In this study, we established the use of FLIC to type seasonal influenza A and B viruses (FLIC-AB). This method has improved sensitivity to 100-fold higher than that of conventional IC methods when we used several strains of influenza viruses. In addition, FLIC-AB demonstrated the ability to detect influenza type A and influenza type B viruses from clinical samples with high sensitivity and specificity (Type A: sensitivity 98.7% (74/75), specificity 100% (54/54), Type B: sensitivity 100% (90/90), specificity 98.2% (54/55) in nasal swab samples) in comparison to the results of qRT-PCR. And furthermore, FLIC-AB performs better in the detection of early stage infection (under 13h) than other conventional IC methods. Our results provide new strategies to prevent the early-stage transmission of influenza viruses in humans during both seasonal outbreaks and pandemics. |
Rights: | https://creativecommons.org/licenses/by/4.0/ |
Type: | article |
URI: | http://hdl.handle.net/2115/75587 |
Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc) 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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