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Fluorescent immunochromatography for rapid and sensitive typing of seasonal influenza viruses

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Title: Fluorescent immunochromatography for rapid and sensitive typing of seasonal influenza viruses
Authors: Sakurai, Akira Browse this author
Takayama, Katsuyoshi Browse this author
Nomura, Namiko Browse this author →KAKEN DB
Kajiwara, Naoki Browse this author →KAKEN DB
Okamatsu, Masatoshi Browse this author →KAKEN DB
Yamamoto, Naoki Browse this author
Tamura, Tsuruki Browse this author
Yamada, Jitsuho Browse this author
Hashimoto, Masako Browse this author
Sakoda, Yoshihiro Browse this author →KAKEN DB
Suda, Yoshihiko Browse this author
Kobayashi, Yukuharu Browse this author
Kida, Hiroshi Browse this author →KAKEN DB
Shibasaki, Futoshi Browse this author →KAKEN DB
Issue Date: 4-Feb-2015
Publisher: Public Library of Science
Journal Title: PLoS One
Volume: 10
Issue: 2
Start Page: e0116715
Publisher DOI: 10.1371/journal.pone.0116715
PMID: 25650570
Abstract: Lateral flow tests also known as Immunochromatography (IC) is an antigen-detection method conducted on a nitrocellulose membrane that can be completed in less than 20 min. IC has been used as an important rapid test for clinical diagnosis and surveillance of influenza viruses, but the IC sensitivity is relatively low (approximately 60%) and the limit of detection (LOD) is as low as 10³ pfu per reaction. Recently, we reported an improved IC assay using antibodies conjugated with fluorescent beads (fluorescent immunochromatography; FLIC) for subtyping H5 influenza viruses (FLIC-H5). Although the FLIC strip must be scanned using a fluorescent reader, the sensitivity (LOD) is significantly improved over that of conventional IC methods. In addition, the antibodies which are specific against the subtypes of influenza viruses cannot be available for the detection of other subtypes when the major antigenicity will be changed. In this study, we established the use of FLIC to type seasonal influenza A and B viruses (FLIC-AB). This method has improved sensitivity to 100-fold higher than that of conventional IC methods when we used several strains of influenza viruses. In addition, FLIC-AB demonstrated the ability to detect influenza type A and influenza type B viruses from clinical samples with high sensitivity and specificity (Type A: sensitivity 98.7% (74/75), specificity 100% (54/54), Type B: sensitivity 100% (90/90), specificity 98.2% (54/55) in nasal swab samples) in comparison to the results of qRT-PCR. And furthermore, FLIC-AB performs better in the detection of early stage infection (under 13h) than other conventional IC methods. Our results provide new strategies to prevent the early-stage transmission of influenza viruses in humans during both seasonal outbreaks and pandemics.
Rights: https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/75587
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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