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Identification of molecular species of phosphatidylcholine hydroperoxides in native and copper-oxidized triglyceride-rich lipoproteins in humans

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/76624

Title: Identification of molecular species of phosphatidylcholine hydroperoxides in native and copper-oxidized triglyceride-rich lipoproteins in humans
Authors: Shrestha, Rojeet Browse this author
Chen, Zhen Browse this author
Miura, Yusuke Browse this author
Yamamoto, Yusuke Browse this author
Sakurai, Toshihiro Browse this author
Chiba, Hitoshi Browse this author →KAKEN DB
Hui, Shu-Ping Browse this author →KAKEN DB
Keywords: Lipid hydroperoxide
oxidation
mass spectrometry
phospholipids
very low-density lipoproteins
intermediate-density lipoproteins
Issue Date: Jan-2020
Publisher: SAGE Publications
Journal Title: Annals of clinical biochemistry
Volume: 57
Issue: 1
Start Page: 95
End Page: 98
Publisher DOI: 10.1177/0004563219880932
PMID: 31537082
Abstract: Background Triglyceride-rich lipoproteins are considered to be independent predictors of atherosclerotic cardiovascular disease. The molecular basis of its atherogenicity is uncertain. Here, we aim to identify molecular species of phosphatidylcholine hydroperoxides (PCOOH) in triglyceride-rich lipoproteins. For comparison, copper-oxidized triglyceride-rich lipoproteins were investigated as well. Methods A fasting EDTA blood sample was collected from six healthy human volunteers to isolate two major triglyceride-rich lipoproteins fractions - very low-density lipoproteins (VLDL) and intermediate-density lipoproteins (IDL) using sequential ultracentrifugation. Triglyceride-rich lipoproteins and plasma samples were studied for PCOOH by liquid chromatography (LC) coupled with Orbitrap mass spectrometry. Results Twelve molecular species of PCOOH in triglyceride-rich lipoproteins and/or plasma were identified using the following criteria: (1) high-resolution mass spectrometry (MS) with mass accuracy within 5 ppm, (2) retention time in LC and (3) fragmentation pattern in MS2 and MS3. PC36:4-OOH was most often detected in VLDL, IDL and plasma. The ratio of total PCOOH to phosphatidylcholine progressively increased with the duration of oxidation in both VLDL and IDL. Conclusion This study demonstrated the presence of 12 molecular species of PCOOH in native triglyceride-rich lipoproteins. The frequent detection of PCOOH in triglyceride-rich lipoproteins provides a molecular basis of the atherogenicity of triglyceride-rich lipoproteins. PCOOH in triglyceride-rich lipoproteins might serve as a molecular basis of the atherogenicity of triglyceride-rich lipoproteins.
Rights: Shrestha, R., Chen, Z., Miura, Y., Yamamoto, Y., Sakurai, T., Chiba, H. and Hui, S.P., Identification of molecular species of phosphatidylcholine hydroperoxides in native and copper-oxidized triglyceride-rich lipoproteins in humans. Annals of clinical biochemistry(57 (1)) pp.95-98.Copyright © 2020 (SAGE Publications). DOI: 10.1177/0004563219880932.
Type: article (author version)
URI: http://hdl.handle.net/2115/76624
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: Rojeet Shrestha

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