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MicroRNA-361-Mediated Inhibition of HSP90 Expression and EMT in Cervical Cancer Is Counteracted by Oncogenic lncRNA NEAT1
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| Title: | MicroRNA-361-Mediated Inhibition of HSP90 Expression and EMT in Cervical Cancer Is Counteracted by Oncogenic lncRNA NEAT1 |
| Authors: | Xu, Daozhi Browse this author | | Dong, Peixin Browse this author →KAKEN DB | | Xiong, Ying Browse this author | | Yue, Junming Browse this author | | Konno, Yosuke Browse this author →KAKEN DB | | Ihira, Kei Browse this author →KAKEN DB | | Kobayashi, Noriko Browse this author | | Todo, Yukiharu Browse this author →KAKEN DB | | Watari, Hidemichi Browse this author →KAKEN DB |
| Keywords: | microRNA-361 | | EMT | | HSP90AA1 | | long non-coding RNA | | NEAT1 | | cervical cancer |
| Issue Date: | 5-Mar-2020 |
| Publisher: | MDPI |
| Journal Title: | Cells |
| Volume: | 9 |
| Issue: | 3 |
| Start Page: | 632 |
| Publisher DOI: | 10.3390/cells9030632 |
| PMID: | 32151082 |
| Abstract: | Epithelial-mesenchymal transition (EMT) is a key process contributing to cervical cancer (CC) metastasis, and microRNAs (miRNAs) modulate the expression of genes implicated in EMT. However, the accurate role of miR-361 in CC-associated EMT and the mechanisms underlying its function in CC remains largely unknown. The functional roles of miR-361 in CC cells were explored by a series of cell functional assays. Luciferase reporter assays were used to demonstrate the potential interaction between miR-361, HSP90, and long non-coding RNA (lncRNA) NEAT1. We detected a reduction of miR-361 expression in CC tissues compared with normal tissues, and miR-361 overexpression inhibited invasion and EMT phenotypes of CC cells by directly targeting a key EMT activator HSP90. Additionally, we detected significantly higher levels of HSP90 in CC tissues compared with normal tissues, and high expression of HSP90 predicted a poorer prognosis. We further identified NEAT1 as a significantly upregulated lncRNA in CC tissues and high expression of NEAT1 was associated with worse survival in CC patients. NEAT1 directly repressed miR-361 expression and played an oncogenic role in CC cell invasion and sphere formation. Conclusions: These results demonstrated that miR-361 directly targets HSP90 to inhibit the invasion and EMT features, and NEAT1 functions as an oncogenic lncRNA that suppresses miR-361 expression and induces EMT and sphere formation in CC cells, thus providing critical insights into the molecular pathways operating in this malignancy. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/76908 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 董 培新
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