|
Hokkaido University Collection of Scholarly and Academic Papers >
Theses >
博士 （歯学） >
Antibacterial coating of human dentin surface with surface pre-reacted glassionomer (S-PRG) nanofillers
|
Files in This Item:
|
|
|
Related Items in HUSCAP:
|
| Title: | Antibacterial coating of human dentin surface with surface pre-reacted glassionomer (S-PRG) nanofillers |
| Other Titles: | surface pre-reacted glassionomer（S-PRG）ナノフィラーによるヒト象牙質表面の抗菌コーティング |
| Authors: | 眞弓, 佳代子 Browse this author |
| Keywords: | Actinomyces naeslundii | | cytotoxicity | | Streptococcus mutans |
| Issue Date: | 25-Mar-2019 |
| Publisher: | Hokkaido University |
| Abstract: | Surface pre-reacted glass ionomer (S-PRG) filler shows tooth remineralization and antibacterial effects via ion-releasing. In this study, we fabricated the nano- and micro-sized S-PRG fillers and assessed the adhesive properties of S-PRG fillers to the human dentin surface. In addition, the antibacterial effects of dentin coated with S-PRG fillers were examined. The stock of S-PRG filler (average particle size; 5 μm) was fractionated to obtain the two types of dispersion; nano and micro scale particle types, and then labeled with nano- and micro-S-PRG, respectively. S-PRG fillers were characterized by scanning electron microscope (SEM), energy dispersive X-ray spectrometry (EDX), ion-releasing test and provided to antibacterial and cytotoxic assessments. Subsequently, we assessed the adhesion test of stock, nano- and micro-S-PRG to human dentin blocks. In addition, antibacterial effects of nano-S-PRG coated dentin was examined using Streptococcus mutans and Actinomyces naeslundii. The results showed that the nano- and micro-S-PRG showed including of elements of F, Na, Al, Si and Sr, ion releasing ability comparable with the stock, antimicrobial activity and low cytotoxicity. SEM and EDX examinations revealed that S-PRG fillers uniformly covered the dentin surface after application of filler to dentin. After ultrasonically cleaning, nano-S-PRG predominantly remained on the dentin surface compared to stock and micro-S-PRG, suggesting that the nano-S-PRG could adhered to dentin. Nano-S-PRG treated dentin showed the sterilization effect and bacterial growth inhibition. In conclusion, nano-S-PRG exhibited great adhesive and antimicrobial properties to human dentin. S-PRG application to tooth would be beneficial for dental antibacterial treatment. |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第13487号 |
| Degree Level: | 博士 |
| Degree Discipline: | 歯学 |
| Examination Committee Members: | (主査) 特任教授 柴田 健一郎, 教授 吉田 靖弘, 准教授 菅谷 勉 |
| Degree Affiliation: | 歯学研究科(口腔医学専攻) |
| Type: | theses (doctoral) |
| URI: | http://hdl.handle.net/2115/77150 |
| Appears in Collections: | 課程博士 (Doctorate by way of Advanced Course) > 歯学院(Graduate School of Dental Medicine)
学位論文 (Theses) > 博士 （歯学）
|
|
