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STAP-2 positively regulates Fc epsilon RI-mediated basophil activation and basophil-dependent allergic inflammatory reactions
| Title: | STAP-2 positively regulates Fc epsilon RI-mediated basophil activation and basophil-dependent allergic inflammatory reactions |
| Authors: | Kashiwakura, Jun-ichi Browse this author →KAKEN DB | | Yamashita, Shinsuke Browse this author | | Yoshihara, Mari Browse this author | | Inui, Kyosuke Browse this author | | Saitoh, Kodai Browse this author | | Sekine, Yuichi Browse this author | | Muromoto, Ryuta Browse this author →KAKEN DB | | Kitai, Yuichi Browse this author →KAKEN DB | | Oritani, Kenji Browse this author | | Matsuda, Tadashi Browse this author →KAKEN DB |
| Keywords: | basophils | | IgE | | IgE-CAI | | IL-4 | | STAP-2 |
| Issue Date: | May-2019 |
| Publisher: | Oxford University Press |
| Journal Title: | International immunology |
| Volume: | 31 |
| Issue: | 5 |
| Start Page: | 349 |
| End Page: | 356 |
| Publisher DOI: | 10.1093/intimm/dxz013 |
| Abstract: | Basophils are an important cell type in the regulation of T(h)2 immune responses. Recently, we revealed that signal-transducing adaptor protein-2 (STAP-2) negatively regulates mast cell activation via Fc epsilon RI. However, the role of STAP-2 in basophil maturation and activation remained unclear. In this study, we demonstrated the normal development of basophils in STAP-2-deficient (STAP-2(-/-)) mice. We also demonstrated in vitro normal basophil differentiation and FceRI expression in STAP2(-/-) mice, suggesting that STAP-2 is dispensable for basophil maturation. Using bone marrow-derived cultured basophils (BMBs), we showed that degranulation and cytokine production of STAP-2(-/-) BMBs were lower than those of wild-type (WT) BMBs upon stimulation with IgE/Ag. In accordance with the reduction of degranulation and cytokine production, phosphorylation of several signal molecules such as Lyn, PLC-gamma 2 and Erk was reduced in STAP-2(-/-) BMBs after stimulation via Fc epsilon RI. Finally, it was observed that IgE-dependent chronic allergic inflammation of STAP-2(-/-) mice was significantly inhibited compared with WT mice. Taken together, we conclude that STAP-2 is an adaptor molecule that positively regulates FceRI-mediated basophil activation and basophil-dependent allergic inflammatory reactions. |
| Rights: | This is a pre-copyedited, author-produced version of an article accepted for publication in International Immunology following peer review. The version of record Jun-ichi Kashiwakura et al. STAP-2 positively regulates FcεRI-mediated basophil activation and basophil-dependent allergic inflammatory reactions. International Immunology, Volume 31, Issue 5, May 2019, Pages 349–356. is available online at: https://doi.org/10.1093/intimm/dxz013 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/78248 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 柏倉 淳一
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