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STAP-2 positively regulates Fc epsilon RI-mediated basophil activation and basophil-dependent allergic inflammatory reactions

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/78248

Title: STAP-2 positively regulates Fc epsilon RI-mediated basophil activation and basophil-dependent allergic inflammatory reactions
Authors: Kashiwakura, Jun-ichi Browse this author →KAKEN DB
Yamashita, Shinsuke Browse this author
Yoshihara, Mari Browse this author
Inui, Kyosuke Browse this author
Saitoh, Kodai Browse this author
Sekine, Yuichi Browse this author
Muromoto, Ryuta Browse this author →KAKEN DB
Kitai, Yuichi Browse this author →KAKEN DB
Oritani, Kenji Browse this author
Matsuda, Tadashi Browse this author →KAKEN DB
Keywords: basophils
IgE
IgE-CAI
IL-4
STAP-2
Issue Date: May-2019
Publisher: Oxford University Press
Journal Title: International immunology
Volume: 31
Issue: 5
Start Page: 349
End Page: 356
Publisher DOI: 10.1093/intimm/dxz013
Abstract: Basophils are an important cell type in the regulation of T(h)2 immune responses. Recently, we revealed that signal-transducing adaptor protein-2 (STAP-2) negatively regulates mast cell activation via Fc epsilon RI. However, the role of STAP-2 in basophil maturation and activation remained unclear. In this study, we demonstrated the normal development of basophils in STAP-2-deficient (STAP-2(-/-)) mice. We also demonstrated in vitro normal basophil differentiation and FceRI expression in STAP2(-/-) mice, suggesting that STAP-2 is dispensable for basophil maturation. Using bone marrow-derived cultured basophils (BMBs), we showed that degranulation and cytokine production of STAP-2(-/-) BMBs were lower than those of wild-type (WT) BMBs upon stimulation with IgE/Ag. In accordance with the reduction of degranulation and cytokine production, phosphorylation of several signal molecules such as Lyn, PLC-gamma 2 and Erk was reduced in STAP-2(-/-) BMBs after stimulation via Fc epsilon RI. Finally, it was observed that IgE-dependent chronic allergic inflammation of STAP-2(-/-) mice was significantly inhibited compared with WT mice. Taken together, we conclude that STAP-2 is an adaptor molecule that positively regulates FceRI-mediated basophil activation and basophil-dependent allergic inflammatory reactions.
Rights: This is a pre-copyedited, author-produced version of an article accepted for publication in International Immunology following peer review. The version of record Jun-ichi Kashiwakura et al. STAP-2 positively regulates FcεRI-mediated basophil activation and basophil-dependent allergic inflammatory reactions. International Immunology, Volume 31, Issue 5, May 2019, Pages 349–356. is available online at: https://doi.org/10.1093/intimm/dxz013
Type: article (author version)
URI: http://hdl.handle.net/2115/78248
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 柏倉 淳一

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