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The presence of anti-neutrophil extracellular trap antibody in patients with microscopic polyangiitis

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/78738

Title: The presence of anti-neutrophil extracellular trap antibody in patients with microscopic polyangiitis
Authors: Hattanda, Fumihiko Browse this author
Nakazawa, Daigo Browse this author →KAKEN DB
Watanabe-Kusunoki, Kanako Browse this author
Kusunoki, Yoshihiro Browse this author
Shida, Haruki Browse this author
Masuda, Sakiko Browse this author →KAKEN DB
Nishio, Saori Browse this author →KAKEN DB
Tomaru, Utano Browse this author →KAKEN DB
Atsumi, Tatsuya Browse this author →KAKEN DB
Ishizu, Akihiro Browse this author →KAKEN DB
Keywords: ANCA
ANCA-associated vasculitis
Neutrophil extracellular traps (NETs)
Anti-NET antibody (ANETA)
Issue Date: 1-Jul-2019
Publisher: Oxford University Press
Journal Title: Rheumatology
Volume: 58
Issue: 7
Start Page: 1293
End Page: 1298
Publisher DOI: 10.1093/rheumatology/kez089
PMID: 30897615
Abstract: Objective Although anti-neutrophil cytoplasmic antibody (ANCA) is the major autoantibody in patients with ANCA-associated vasculitis (AAV), previous studies have suggested the presence of anti-neutrophil extracellular trap (NET) antibody in patients with microscopic polyangiitis (MPA), one type of AAV. In this study, we aimed to determine the prevalence and pathogenic role of anti-NET antibody (ANETA) in MPA. Methods We examined the presence or absence of ANETA in sera obtained from 19 MPA patients by indirect immunofluorescence. We compared the clinical parameters, including age, sex, myeloperoxidase (MPO)-ANCA, creatinine, C-reactive protein, MPO-DNA complexes, and vasculitis activity, in ANETA-positive and ANETA-negative MPA patients. We investigated the serum NET induction and degradation abilities of ANETA-positive and ANETA-negative MPA patients with reference to healthy controls (n=8). Furthermore, we assessed the relationship of ANETA with the effect of IgG depletion on the serum NET degradation ability. Results ANETA was present in 10 of the 19 MPA patients. There was no significant difference in the clinical parameters in ANCA-positive and ANCA-negative MPA patients. Although the NET induction ability was higher and the NET degradation ability was lower in MPA sera than those in healthy controls, these abilities were not different between ANETA-positive and ANETA-negative MPA sera. Interestingly, the NET degradation ability in some sera with ANETA was markedly increased by IgG depletion. Conclusion Some MPA patients produce ANETA and some ANETA possess an inhibitory function against the serum NET degradation ability. Although further studies are needed, ANETA is worthy of attention for understanding the pathophysiology of MPA.
Rights: This is a pre-copyedited, author-produced version of an article accepted for publication in Rheumatology following peer review. The version of record Fumihiko Hattanda, Daigo Nakazawa, Kanako Watanabe-Kusunoki, Yoshihiro Kusunoki, Haruki Shida, Sakiko Masuda, Saori Nishio, Utano Tomaru, Tatsuya Atsumi, Akihiro Ishizu, The presence of anti-neutrophil extracellular trap antibody in patients with microscopic polyangiitis, Rheumatology, Volume 58, Issue 7, July 2019, Pages 1293–1298 is available online at: https://doi.org/10.1093/rheumatology/kez089.
Type: article (author version)
URI: http://hdl.handle.net/2115/78738
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石津 明洋

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