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Histopathological changes in tear-secreting tissues and cornea in a mouse model of autoimmune disease

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Title: Histopathological changes in tear-secreting tissues and cornea in a mouse model of autoimmune disease
Authors: Masaya, Hiraishi Browse this author
Md Abdul, Masum Browse this author
Takashi, Namba Browse this author
Otani, Yuki Browse this author
Elewa, Yaser H. A. Browse this author
Ichii, Osamu Browse this author →KAKEN DB
Kon, Yasuhiro Browse this author
Keywords: Tear film
autoimmune disease
MpJ-Yaa mice
Issue Date: 1-Jun-2020
Publisher: SAGE Publications
Journal Title: Experimental Biology and Medicine
Volume: 245
Issue: 12
Start Page: 999
End Page: 1008
Publisher DOI: 10.1177/1535370220928275
Abstract: The tear film covers the cornea, and its abnormalities (including immunological) induce dry eye. Using autoimmune disease model mice, BXSB/MpJ-Yaa (BXSB-Yaa), histopathological changes in the eye and tear-secreting tissues were examined using histopathology, immunohistochemistry, and electron microscopy at 8, 20, and 28 weeks for early, middle, and late disease stages. Early and middle stage BXSB-Yaa showed increased serum autoantibody and spleen weight-to-body weight (S/B) ratio, respectively, and higher tear volume than controls, BXSB/MpJ (BXSB), at early stages, which decreased with ageing and negatively correlated with autoimmune disease indices. Smaller Meibomian gland acini, intraorbital lacrimal glands, and Harderian gland acinar cells were seen in late stage BXSB-Yaa than in BXSB; the latter two indices decreased with ageing and negatively correlated with the S/B ratio. Cell infiltration occurred in the middle stage BXSB-Yaa extraorbital lacrimal gland, and acinar cells were smaller than BXSB. The conjunctival goblet cells decreased from early to middle stages in both strains, but in BXSB-Yaa, they increased at late stages with a partial lack of microvilli on the cornea and were inversely altered with anterior epithelium thickness through ageing, suggesting that they compensated for anterior epithelium damage. In conclusion, the tear film was unstable due to an autoimmune disease condition in BXSB-Yaa. Impact statement Cornea, an outermost layer of mammalian eye, is protected by tear film and abnormalities of tear film causes dry eye. Dry eye injures the cornea which results lower vision in patients. Several factors cause dry eye, including altered systemic conditions, environment, and immunological abnormality of the patient in autoimmune disease like Sjogren's syndrome (SS). However, the detailed pathology of autoimmune abnormality-mediated dry eye is unclear. Here we demonstrated that systemic autoimmune abnormality in BXSB-Yaa mice was associated with histological changes in the exocrine glands and cornea of the eyes. We also showed that BXSB-Yaa mice developed mild or early stage dry eye-like disease and explain the existence of a compensatory mechanism associated with the dysfunction of these tissues. Thus, BXSB-Yaa could be a model for SS-like disease-associated dry eye and these data would contribute to the understanding of the pathogenesis of autoimmune-related dry eye disease.
Rights: Masaya Hiraishi, Md Abdul Masum, Takashi Namba, Yuki Otani, Yaser HA Elewa, Osamu Ichii, Yasuhiro Kon, Histopathological changes in tear-secreting tissues and cornea in a mouse model of autoimmune disease, Experimental Biology and Medicine (Volume 245 Issue 12) pp. 999-1008. Copyright © 2020 the Society for Experimental Biology and Medicine (SEBM). DOI: 10.1177/1535370220928275
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 市居 修

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