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Hokkaido University Collection of Scholarly and Academic Papers >
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Optimization of Extraction of Gelatin from the Head of Kalamtra Sturgeon (Huso dauricus x Acipenser scherenkii x Acipenser transmontanus)
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| Title: | Optimization of Extraction of Gelatin from the Head of Kalamtra Sturgeon (Huso dauricus x Acipenser scherenkii x Acipenser transmontanus) |
| Authors: | Islam, Md. Rashidul Browse this author | | Yuhi, Tomoharu Browse this author | | Ura, Kazuhiro Browse this author →KAKEN DB | | Takagi, Yasuaki Browse this author →KAKEN DB |
| Keywords: | sturgeon head | | tissue separation | | pretreatment | | extraction conditions |
| Issue Date: | Oct-2020 |
| Publisher: | MDPI |
| Journal Title: | Applied sciences |
| Volume: | 10 |
| Issue: | 19 |
| Start Page: | 6660 |
| Publisher DOI: | 10.3390/app10196660 |
| Abstract: | To develop a new method for extracting gelatin from the sturgeon head, the conditions for pretreatment and extraction were optimized. Treatment at 65 degrees C (3-3.5 h) was enough to separate the head into mixed tissues (skin, scales, pectoral fins, muscle, bones, gills, and small cartilage pieces), skull cartilage, and liquid. From the intensities of alpha-, beta- and gamma-bands and yields, the optimized pretreatment conditions for type A and type B gelatin were 0.05 M HCl (1 h) and 0.1 M NaOH (1 h), respectively. The best extraction conditions were: (1) for type A gelatin: with distilled water (DW) (w/v 1:5) at 35 degrees C, pH 7 when stirring at 200 rpm for 3 h, and (2) for type B gelatin: with DW (w/v 1:5) at 50 degrees C, pH 8 when stirring at 200 rpm for 1 h. After the decalcification of extracted residues with 0.05 M HCl for 3 h, re-extraction of gelatin was possible. Under the best extraction conditions, yields of type A and B gelatins were 5.01 and 7.25% (dry gelatin weight/wet sample weight), respectively. Thus, it is possible to extract an industrial amount of gelatin from sturgeon heads, making them valuable by-products. |
| Rights: | © 2020 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution License. | | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/79956 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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