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Potential of cell-free DNA as a screening marker for parasite infections in dog

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Title: Potential of cell-free DNA as a screening marker for parasite infections in dog
Authors: Akter, Shirin Browse this author
Nakao, Ryo Browse this author →KAKEN DB
Imasato, Yuhei Browse this author
Alam, Mohammad Zahangir Browse this author
Katakura, Ken Browse this author
Keywords: Babesia
Cell-free DNA
Parasitic infections
Stray dogs
Issue Date: Jul-2019
Publisher: Elsevier
Journal Title: Genomics
Volume: 111
Issue: 4
Start Page: 906
End Page: 912
Publisher DOI: 10.1016/j.ygeno.2018.05.020
Abstract: Parasitic infections are common in stray dogs and accurate knowledge of parasite communities in dogs would provide insight into the epidemiology of parasitic diseases. In this study, we used Illumina sequencing technology to evaluate cell-free DNA (cfDNA) as a marker for screening of parasitic infections in dogs. Plasma samples from 14 stray dogs captured in Bangladesh were used in the experiments. An average of 2.3 million reads was obtained for each sample. BLASTn analysis identified 150 reads with high similarity with parasites from 19 different genera. In particular, we detected sequences of Babesia spp. in five dogs; consistent with this, a previous study using conventional PCR showed that four of these dogs were positive for B. gibsoni. Several reads with similarity to Leishmania and filarial nematodes were also identified. These findings indicate that cfDNA in blood can be a potential screening marker for identifying parasite diversity in dogs.
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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