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Characterization and antibacterial assessments of Ag nanoclusters/rose bengal nanocomposite for antimicrobial photodynamic therapy

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Please use this identifier to cite or link to this item:https://doi.org/10.14943/doctoral.k13482
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Title: Characterization and antibacterial assessments of Ag nanoclusters/rose bengal nanocomposite for antimicrobial photodynamic therapy
Other Titles: 抗菌的光線力学療法に向けた銀ナノクラスター/ローズベンガル複合体の特性並びに抗菌性評価
Authors: 蔀, 佳奈子 Browse this author
Keywords: Aggregatibacter actinomycetemcomitans
Photosensitizer
Porphyromonas gingivalis
Singlet oxygen
Streptococcus mutans
Issue Date: 25-Mar-2019
Publisher: Hokkaido University
Abstract: Ag nanoclusters (AgNCs) / rose bengal (RB) nanocomposite (AgNCs/RB) as a novel photosensitizer was created for antibacterial photodynamic therapy (a-PDT). We proposed double antibacterial effects of photoexcited AgNCs/RB; (1) singlet oxygen (1O2) generated by irradiated RB and (2) Ag+ ion released by AgNCs oxidized by 1O2. In present study, the antimicrobial and the cytotoxic effects of AgNCs/RB were evaluated.1O2 generation, zeta potential and Ag+ release of AgNCs/RB was characterized. Subsequently, the antimicrobial effect of AgNCs/RB irradiated by white light-emitting diode (LED) was evaluated using oral bacterial cells; Streptococcus mutans, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. In addition, cytotoxicity of AgNCs/RB on NIH3T3 mammalian cells was investigated. The binding of AgNCs and RB, 1O2 generation and Ag+ ion release of photoexcited AgNCs/RB were estimated. AgNCs/RB irradiated by white LED (60 sec) showed significantly inhibited the growth of oral bacteria when compared to AgNCs or RB. In addition, photoexcited AgNCs/RB did not negatively affect the adhesion, spreading and proliferation of mammalian cells, even at concentrations that showed antibacterial activity. In conclusion, AgNCs/RB irradiated by white LED showed great antimicrobial effects via 1O2 generation and Ag+ ion releasing and low cytotoxic properties to be provided for dental a-PDT.
Conffering University: 北海道大学
Degree Report Number: 甲第13482号
Degree Level: 博士
Degree Discipline: 歯学
Examination Committee Members: (主査) 特任教授 柴田 健一郎, 教授 田村 正人, 准教授 菅谷 勉
Degree Affiliation: 歯学研究科(口腔医学専攻)
Type: theses (doctoral)
URI: http://hdl.handle.net/2115/80729
Appears in Collections:課程博士 (Doctorate by way of Advanced Course) > 歯学院(Graduate School of Dental Medicine)
学位論文 (Theses) > 博士 (歯学)

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