HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
Peer-reviewed Journal Articles, etc >

Antibacterial and Antibiofilm Photodynamic Activities of Lysozyme-Au Nanoclusters/Rose Bengal Conjugates

Files in This Item:

The file(s) associated with this item can be obtained from the following URL:

Title: Antibacterial and Antibiofilm Photodynamic Activities of Lysozyme-Au Nanoclusters/Rose Bengal Conjugates
Authors: Okamoto, Ichie Browse this author
Miyaji, Hirofumi Browse this author →KAKEN DB
Miyata, Saori Browse this author
Shitomi, Kanako Browse this author →KAKEN DB
Sugaya, Tsutomu Browse this author →KAKEN DB
Ushijima, Natsumi Browse this author
Akasaka, Tsukasa Browse this author →KAKEN DB
Enya, Satoshi Browse this author
Saita, Satoshi Browse this author
Kawasaki, Hideya Browse this author →KAKEN DB
Issue Date: 6-Apr-2021
Publisher: American Chemical Society
Journal Title: ACS Omega
Volume: 6
Issue: 13
Start Page: 9279
End Page: 9290
Publisher DOI: 10.1021/acsomega.1c00838
PMID: 33842797
Abstract: Antibacterial photodynamic therapy (aPDT) utilizes reactive oxygen species such as singlet oxygen (O-1(2)) and free radicals via photosensitizers, which are light and light-sensitive agents, to reduce bacterial infections. It has been utilized as a treatment for dental diseases in place of antibiotic therapies. However, aPDT does not always cause the desired therapeutic effect due to the instability of organic photosensitizers and the formation of bacterial biofilms. To promote the antibacterial and antibiofilm effects of aPDT, we have proposed a lysozyme (Lys)-gold nanoclusters (Au NCs)/rose bengal (Lys-Au NCs/RB) conjugate as a novel photosensitizer. This conjugate was found to effectively impede the growth of both gram-positive and gram-negative bacteria when exposed to white light-emitting diode (LED) irradiation. The photoexcited Lys-Au NCs/RB showed significantly higher antibacterial activity than photoexcited Lys-Au NCs or RB alone. The synergistic effect is a result of the combination of Lys (an antibacterial protein) and enhanced O-1(2) generation related to resonance energy transfer (RET) in the Au NCs/RB conjugate. Photoexcited Lys-Au NCs/RB increased the effects of aPDT in a dose- and time-dependent manner. Furthermore, the photoexcited Lys-Au NCs/RB successfully decreased Streptococcus mutans biofilm formation. However, in contrast, it did not have a negative effect on the proliferation, adhesion, or spread of mammalian cells, indicating low cytotoxicity. Lys-Au NCs/RB is a novel photosensitizer with low cytotoxicity that is capable of bacterial inactivation and the suppression of biofilm formation, and could help to improve dental treatments in the future.
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 - Hokkaido University