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Abundant Synthesis of Netrin-1 in Satellite Cell-Derived Myoblasts Isolated from EDL Rather Than Soleus Muscle Regulates Fast-Type Myotube Formation

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Title: Abundant Synthesis of Netrin-1 in Satellite Cell-Derived Myoblasts Isolated from EDL Rather Than Soleus Muscle Regulates Fast-Type Myotube Formation
Authors: Suzuki, Takahiro Browse this author
Mori, Aika Browse this author
Maeno, Takahiro Browse this author
Arimatsu, Rio Browse this author
Ichimura, Emi Browse this author
Nishi, Yuriko Browse this author
Hisaeda, Kouga Browse this author
Yamaya, Yuki Browse this author
Kobayashi, Ken Browse this author →KAKEN DB
Nakamura, Mako Browse this author
Tatsumi, Ryuichi Browse this author
Ojima, Koichi Browse this author
Nishimura, Takanori Browse this author →KAKEN DB
Keywords: satellite cells
myofiber type
myosin heavy chain
Issue Date: May-2021
Publisher: MDPI
Journal Title: International Journal of Molecular Sciences
Volume: 22
Issue: 9
Start Page: 4499
Publisher DOI: 10.3390/ijms22094499
Abstract: Resident myogenic stem cells (satellite cells) are attracting attention for their novel roles in myofiber type regulation. In the myogenic differentiation phase, satellite cells from soleus muscle (slow fiber-abundant) synthesize and secrete higher levels of semaphorin 3A (Sema3A, a multifunctional modulator) than those derived from extensor digitorum longus (EDL; fast fiber-abundant), suggesting the role of Sema3A in forming slow-twitch myofibers. However, the regulatory mechanisms underlying fast-twitch myotube commitment remain unclear. Herein, we focused on netrin family members (netrin-1, -3, and -4) that compete with Sema3A in neurogenesis and osteogenesis. We examined whether netrins affect fast-twitch myotube generation by evaluating their expression in primary satellite cell cultures. Initially, netrins are upregulated during myogenic differentiation. Next, we compared the expression levels of netrins and their cell membrane receptors between soleus- and EDL-derived satellite cells; only netrin-1 showed higher expression in EDL-derived satellite cells than in soleus-derived satellite cells. We also performed netrin-1 knockdown experiments and additional experiments with recombinant netrin-1 in differentiated satellite cell-derived myoblasts. Netrin-1 knockdown in myoblasts substantially reduced fast-type myosin heavy chain (MyHC) expression; exogenous netrin-1 upregulated fast-type MyHC in satellite cells. Thus, netrin-1 synthesized in EDL-derived satellite cells may promote myofiber type commitment of fast muscles.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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