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Propolis suppresses cytokine production in activated basophils and basophil-mediated skin and intestinal allergic inflammation in mice
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| Title: | Propolis suppresses cytokine production in activated basophils and basophil-mediated skin and intestinal allergic inflammation in mice |
| Authors: | Kashiwakura, Jun-ichi Browse this author →KAKEN DB | | Yoshihara, Mari Browse this author | | Saitoh, Kodai Browse this author | | Kagohashi, Kota Browse this author | | Sasaki, Yuto Browse this author | | Kobayashi, Fuki Browse this author | | Inagaki, Iori Browse this author | | Kitai, Yuichi Browse this author →KAKEN DB | | Muromoto, Ryuta Browse this author →KAKEN DB | | Matsuda, Tadashi Browse this author →KAKEN DB |
| Keywords: | Basophils | | Food allergy | | IgE | | IL-4 | | Propolis |
| Issue Date: | Jul-2021 |
| Publisher: | Elsevier |
| Journal Title: | Allergology International |
| Volume: | 70 |
| Issue: | 3 |
| Start Page: | 360 |
| End Page: | 367 |
| Publisher DOI: | 10.1016/j.alit.2020.11.005 |
| Abstract: | Background
Propolis is a resinous mixture produced by honey bees that contains cinnamic acid derivatives and flavonoids. Although propolis has been reported to inhibit mast cell functions and mast cell-dependent allergic responses, the effect of propolis on basophil biology remains unknown. This study aimed to investigate the inhibitory effect of propolis on FcεRI-mediated basophil activation.
Methods
To determine the inhibitory effect of propolis on basophil activation in vitro, cytokine production and FcεRI signal transduction were analyzed by ELISA and western blotting, respectively. To investigate the inhibitory effect of propolis in vivo, IgE-CAI and a food allergy mouse model were employed.
Results
Propolis treatment resulted in the suppression of IgE/antigen-induced production of IL-4, IL-6 and IL-13 in basophils. Phosphorylation of FcεRI signaling molecules Lyn, Akt and ERK was inhibited in basophils treated with propolis. While propolis did not affect the basophil population in the treated mice, propolis did inhibit IgE-CAI. Finally, ovalbumin-induced intestinal anaphylaxis, which involves basophils and basophil-derived IL-4, was attenuated in mice prophylactically treated with propolis.
Conclusions
Taken together, these results demonstrate the ability of propolis to suppress IgE-dependent basophil activation and basophil-dependent allergic inflammation. Therefore, prophylactic treatment with propolis may be useful for protection against food allergic reactions in sensitive individuals. |
| Rights: | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/82067 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松田 正
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