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Developmental potential of somatic and germ cells of hybrids between Carassius auratus females and Hemigrammocypris rasborella males

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Title: Developmental potential of somatic and germ cells of hybrids between Carassius auratus females and Hemigrammocypris rasborella males
Authors: Naya, Yuki Browse this author
Matsunaga, Tomoka Browse this author
Shimizu, Yu Browse this author
Takahashi, Eisuke Browse this author
Shima, Fumika Browse this author
Endoh, Mitsuru Browse this author
Fujimoto, Takafumi Browse this author →KAKEN DB
Arai, Katsutoshi Browse this author →KAKEN DB
Yamaha, Etsuro Browse this author →KAKEN DB
Keywords: Chimera
Issue Date: Dec-2020
Publisher: Cambridge University Press
Journal Title: Zygote
Volume: 28
Issue: 6
Start Page: 470
End Page: 481
Publisher DOI: 10.1017/S0967199420000349
Abstract: The cause of hybrid sterility and inviability has not been analyzed in the fin-fish hybrid, although large numbers of hybridizations have been carried out. In this study, we produced allo-diploid hybrids by cross-fertilization between female goldfish (Carassius auratus) and male golden venus chub (Hemigrammocypris rasborella). Inviability of these hybrids was due to breakage of the enveloping layer during epiboly or due to malformation with serious cardiac oedema around the hatching stage. Spontaneous allo-triploid hybrids with two sets of the goldfish genome and one set of the golden venus chub genome developed normally and survived beyond the feeding stage. This improved survival was confirmed by generating heat-shock-induced allo-triploid hybrids that possessed an extra goldfish genome. When inviable allo-diploid hybrid cells were transplanted into goldfish host embryos at the blastula stage, these embryos hatched normally, incorporating the allo-diploid cells. These allo-diploid hybrid cells persisted, and were genetically detected in a 6-month-old fish. In contrast, primordial germ cells taken from allo-diploid hybrids and transplanted into goldfish hosts at the blastula stage had disappeared by 10 days post-fertilization, even under chimeric conditions. In allo-triploid hybrid embryos, germ cells proliferated in the gonad, but had disappeared by 10 weeks post-fertilization. These results showed that while hybrid germ cells are inviable even in chimeric conditions, hybrid somatic cells remain viable.
Rights: This article has been published in a revised form in Zygote This version is published under a Creative Commons CC-BY-NC-ND. No commercial re-distribution or re-use allowed. Derivative works cannot be distributed. © The Author(s), 2020. Published by Cambridge University Press.
Type: article
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山羽 悦郎

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