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Quantification of Phytophthora infestans population densities and their changes in potato field soil using real-time PCR

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Title: Quantification of Phytophthora infestans population densities and their changes in potato field soil using real-time PCR
Authors: Osawa, Hisashi Browse this author
Suzuki, Nobuyuki Browse this author
Akino, Seishi Browse this author →KAKEN DB
Araki, Hiromichi Browse this author
Asano, Kenji Browse this author →KAKEN DB
Akai, Kotaro Browse this author
Kondo, Norio Browse this author →KAKEN DB
Issue Date: 18-Mar-2021
Publisher: Nature Research
Journal Title: Scientific reports
Volume: 11
Issue: 1
Start Page: 6266
Publisher DOI: 10.1038/s41598-021-85492-z
Abstract: Tuber infection of Phytophthora infestans often occurs at harvest. However, it is difficult to accurately estimate the population densities of P. infestans in soil, especially Japanese soil. In the present study, P. infestans DNA was extracted from soil samples using a modified CTAB-bead method and quantified using real-time PCR to accurately, rapidly and easily estimate the P. infestans population densities in upland soils in Japan. P. infestans was well quantified in eleven types of soil samples, including nine types of upland soils in Japan, that were artificially inoculated with a zoosporangia suspension. The amounts of P. infestans DNA estimated by the real-time PCR were proportional to the inoculum densities. In the non-controlled experimental potato field, P. infestans population densities in soil corresponded to the development of symptoms and were correlated with the number of lesions on the potato foliage. These results imply that the proposed real-time PCR assay is suitable for the estimation or monitoring of P. infestans population densities in upland soils in Japan. The population densities at the ridge bottoms were larger than those at any other location in commercial potato fields. These results were similar to those of a previous report using a bioassay. Moreover, a correlation between DNA quantity and inoculum potential was observed. In conclusion, the real-time PCR assay developed in this study is suitable for indirect estimation of the inoculum potential of P. infestans.
Type: article
URI: http://hdl.handle.net/2115/82430
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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