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PenA, a penicillin-binding protein-type thioesterase specialized for small peptide cyclization

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Title: PenA, a penicillin-binding protein-type thioesterase specialized for small peptide cyclization
Authors: Matsuda, Kenichi Browse this author →KAKEN DB
Fujita, Kei Browse this author
Wakimoto, Toshiyuki Browse this author →KAKEN DB
Keywords: Cyclopeptide
Issue Date: 13-Mar-2021
Publisher: Oxford University Press
Journal Title: Journal of industrial microbiology and biotechnology
Volume: 48
Issue: 3-4
Start Page: kuab023
Publisher DOI: 10.1093/jimb/kuab023
Abstract: Penicillin-binding protein-type thioesterases (PBP-type TEs) are a recently identified group of peptide cyclases that catalyze head-to-tail macrolactamization of nonribosomal peptides. PenA, a new member of this group, is involved in the biosyntheses of cyclic pentapeptides. In this study, we demonstrated the enzymatic activity of PenA in vitro, and analyzed its substrate scope with a series of synthetic substrates. A comparison of the reaction profiles between PenA and SurE, a representative PBP-type TE, showed that PenA is more specialized for small peptide cyclization. A computational model provided a possible structural rationale for the altered specificity for substrate chain lengths.
Type: article
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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