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Binding of collagen gene products with titanium oxide

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Title: Binding of collagen gene products with titanium oxide
Authors: Song, Qin Browse this author
Iku, Shouhei Browse this author
Sammons, Rachel Browse this author
Yagami, Kimitoshi Browse this author →KAKEN DB
Furusawa, Toshitake Browse this author
Morimoto, Koichi Browse this author →KAKEN DB
Rahaman, Md Shiblur Browse this author
Kurasaki, Masaaki Browse this author →KAKEN DB
Tokura, Seiichi Browse this author →KAKEN DB
Kuboki, Yoshinori Browse this author →KAKEN DB
Keywords: anatase
titanium beads
2M urea
Issue Date: May-2021
Publisher: Oxford University Press
Journal Title: Journal of Biochemistry
Volume: 169
Issue: 5
Start Page: 565
End Page: 573
Publisher DOI: 10.1093/jb/mvaa146
Abstract: Titanium is the only metal to which osteoblasts can adhere and on which they can grow and form bone tissue in vivo, resulting in a strong bond between the implant and living bone. This discovery provides the basis for the universal medical application of Ti. However, the biochemical mechanism of bond formation is still unknown. We aimed to elucidate the mechanism of bond formation between collagen, which constitutes the main organic component of bone, and TiO2, of which the entire surface of pure Ti is composed. We analysed the binding between the soluble collagen and TiO2 by chromatography with a column packed with Ti beads of 45 mu m, and we explored the association between collagen fibrils and TiO2 (anatase) powders of 0.2 mu m. We ran the column of chromatography under various elution conditions. We demonstrated that there is a unique binding affinity between Ti and collagen. This binding capacity was not changed even in the presence of the dissociative solvent 2M urea, but it decreased after heat denaturation of collagen, suggesting the contribution of the triple-helical structure. We propose a possible role of periodically occurring polar amino acids and the collagen molecules in the binding with TiO2.
Type: article
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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