HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Health Sciences / Faculty of Health Sciences >
Peer-reviewed Journal Articles, etc >

Analysis of serum lysophosphatidylethanolamine levels in patients with non-alcoholic fatty liver disease by liquid chromatography-tandem mass spectrometry

Files in This Item:
yamamoto2020.pdf474.91 kBPDFView/Open
Please use this identifier to cite or link to this item:

Title: Analysis of serum lysophosphatidylethanolamine levels in patients with non-alcoholic fatty liver disease by liquid chromatography-tandem mass spectrometry
Authors: Yamamoto, Yusuke Browse this author
Sakurai, Toshihiro Browse this author
Chen, Zhen Browse this author →KAKEN DB
Furukawa, Takayuki Browse this author →KAKEN DB
Gowda, Siddabasave Gowda B. Browse this author
Wu, Yue Browse this author
Nouso, Kazuhiro Browse this author →KAKEN DB
Fujii, Yuki Browse this author
Yoshikawa, Yuki Browse this author
Chiba, Hitoshi Browse this author →KAKEN DB
Hui, Shu-Ping Browse this author →KAKEN DB
Keywords: Lysophosphatidylethanolamine
Liquid chromatography-tandem mass spectrometry
Chemical synthesis
Non-alcoholic fatty liver disease
Issue Date: 22-Oct-2020
Publisher: Springer
Journal Title: Analytical and bioanalytical chemistry
Volume: 413
Issue: 1
Start Page: 245
End Page: 254
Publisher DOI: 10.1007/s00216-020-02996-9
PMID: 33090255
Abstract: Lysophosphatidylethanolamines (LysoPEs) are the partial hydrolysis products of phosphatidylethanolamine. Despite the unique in vitro bioactivities of LysoPEs, there are limited reports on the pathophysiological role of LysoPEs in the serum, due to the lack of sensitive analytical methods for determination of each molecular species in clinical samples. Herein, we developed a highly sensitive quantitative method to profile the serum LysoPE species by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with selected reaction monitoring (SRM). The internal standard (IS), chemically synthesized in-house, and the lineup of seven major LysoPE species were used in this study. The limits of detection and quantification for each LysoPE species ranged within 0.5-3.3 pmol/mL and 1.0-5.0 pmol/mL, respectively. The combined concentrations of LysoPEs in the serum from healthy subjects (n = 8) and the patients with non-alcoholic fatty liver diseases (NAFLD) including simple steatosis (SS, n = 9) and non-alcoholic steatohepatitis (NASH, n = 27) were 18.030 +/- 3.832, 4.867 +/- 1.852, and 5.497 +/- 2.495 nmol/mL, respectively. The combined and individual concentrations of LysoPEs, except for LysoPE 18:0, significantly decreased in the patients with NAFLD compared with those for the healthy subjects. However, no significant difference was observed between the SS and NASH groups. Our proposed LC-MS/MS method is valid and has advantages of small sample volume, high sensitivity, and simultaneous absolute quantitation for multiple molecular species. This method may enable diagnostic evaluation and elucidation of the as-yet uncovered pathophysiological role of LysoPEs.
Rights: This is a post-peer-review, pre-copyedit version of an article published in Analytical and bioanalytical chemistry. The final authenticated version is available online at:
Type: article (author version)
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 惠 淑萍

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 - Hokkaido University